| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COG1 |
| Uniprot No | Q8WTW3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-980aa |
| 氨基酸序列 | MATAATSPALKRLDLRDPAALFETHGAEEIRGLERQVRAEIEHKKEELRQMVGERYRDLIEAADTIGQMRRCAVGLVDAVKATDQYCARLRQAGSAAPRPPRAQQPQQPSQEKFYSMAAQIKLLLEIPEKIWSSMEASQCLHATQLYLLCCHLHSLLQLDSSSSRYSPVLSRFPILIRQVAAASHFRSTILHESKMLLKCQGVSDQAVAEALCSIMLLEESSPRQALTDFLLARKATIQKLLNQPHHGAGIKAQICSLVELLATTLKQAHALFYTLPEGLLPDPALPCGLLFSTLETITGQHPAGKGTGVLQEEMKLCSWFKHLPASIVEFQPTLRTLAHPISQEYLKDTLQKWIHMCNEDIKNGITNLLMYVKSMKGLAGIRDAMWELLTNESTNHSWDVLCRRLLEKPLLFWEDMMQQLFLDRLQTLTKEGFDSISSSSKELLVSALQELESSTSNSPSNKHIHFEYNMSLFLWSESPNDLPSDAAWVSVANRGQFASSGLSMKAQAISPCVQNFCSALDSKLKVKLDDLLAYLPSDDSSLPKDVSPTQAKSSAFDRYADAGTVQEMLRTQSVACIKHIVDCIRAELQSIEEGVQGQQDALNSAKLHSVLFMARLCQSLGELCPHLKQCILGKSESSEKPAREFRALRKQGKVKTQEIIPTQAKWQEVKEVLLQQSVMGYQVWSSAVVKVLIHGFTQSLLLDDAGSVLATATSWDELEIQEEAESGSSVTSKIRLPAQPSWYVQSFLFSLCQEINRVGGHALPKVTLQEMLKSCMVQVVAAYEKLSEEKQIKKEGAFPVTQNRALQLLYDLRYLNIVLTAKGDEVKSGRSKPDSRIEKVTDHLEALIDPFDLDVFTPHLNSNLHRLVQRTSVLFGLVTGTENQLAPRSSTFNSQEPHNILPLASSQIRFGLLPLSMTSTRKAKSTRNIETKAQVVPPARSTAGDPTVPGSLFRQLVSEEDNTSAPSLFKLGWLSSMTK |
| 分子量 | 135.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人COG1蛋白的参考文献信息(内容基于现有知识推测整理，建议通过学术数据库验证具体文献)：
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1. **文献名称**: *The role of COG1 in Golgi complex integrity and glycosylation*
**作者**: Smith J, et al.
**摘要**: 探讨COG1蛋白作为寡聚高尔基体复合体(COG complex)核心成分的功能，研究发现重组人COG1蛋白通过与COG3等亚基互作维持高尔基体结构，其缺陷导致蛋白质糖基化异常，与先天性糖基化疾病相关。
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2. **文献名称**: *Recombinant human COG1 production and its interaction with Rab GTPases*
**作者**: Chen L, et al.
**摘要**: 报道重组人COG1蛋白在大肠杆菌中的表达与纯化方法，并验证其与Rab GTPase(如Rab1和Rab6)的体外结合活性，表明COG1在囊泡运输中的调控作用依赖此类互作。
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3. **文献名称**: *COG1 mutations disrupt ER-Golgi trafficking and cause developmental disorders*
**作者**: Wang Y, et al.
**摘要**: 分析COG1基因突变患者的细胞模型，发现突变导致重组COG1蛋白功能丧失，引发内质网-高尔基体运输障碍，临床表现为神经发育异常与多器官缺陷，提示其治疗需靶向COG复合体修复。
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**注意**：以上文献信息为示例，实际研究中需通过PubMed、Google Scholar等平台以关键词“recombinant human COG1”、“COG complex”或“COG1 glycosylation”检索真实文献。
**Background of Recombinant Human COG1 Protein**
The Conserved Oligomeric Golgi (COG) complex, a critical player in intracellular vesicle trafficking and Golgi apparatus maintenance, comprises eight subunits (COG1-8) arranged into two subcomplexes. COG1. a core component of the COG complex's lobe A (alongside COG2-4), is essential for tethering retrograde vesicles to the Golgi, ensuring proper glycosylation, protein sorting, and organelle integrity. Mutations in COG1 are linked to congenital disorders of glycosylation (CDGs), characterized by developmental delays, neurological deficits, and multisystem abnormalities due to impaired protein and lipid glycosylation.
Recombinant human COG1 (rhCOG1) is engineered via molecular cloning and expression in heterologous systems (e.g., mammalian cells or bacteria) to produce functional, high-purity protein for research. Its production enables detailed studies on COG1's structural-functional relationships, interactions with other COG subunits, and mechanisms underlying COG-related pathologies. rhCOG1 is instrumental in developing cellular models to dissect Golgi dysfunction, screen therapeutic compounds, and explore gene therapy approaches for COG1-deficient disorders. By providing a controlled, scalable source of COG1. recombinant technology advances both basic research and translational efforts targeting glycosylation-related diseases.
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