| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COBRA1 |
| Uniprot No | Q8WX92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ LDHRKPSPAQ AAETPALELP LPSVPAPAPL |
| 分子量 | 65.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COBRA1蛋白的3篇文献示例(部分内容为模拟概括，实际需根据具体文献调整)：
1. **文献名称**：*COBRA1. a transcriptional regulator involved in human development and disease*
**作者**：Smith J, et al.
**摘要**：本文探讨COBRA1蛋白在哺乳动物胚胎发育中的作用，通过重组蛋白实验揭示其与RNA聚合酶II复合物的相互作用，并证明其缺失导致胚胎干细胞分化异常。
2. **文献名称**：*Structural and functional analysis of the human COBRA1 protein*
**作者**：Li X, et al.
**摘要**：利用重组COBRA1蛋白进行晶体结构解析，发现其N端结构域与转录共激活因子BRCA1存在直接结合，提示其在DNA损伤修复通路中的潜在调控作用。
3. **文献名称**：*COBRA1 modulates transcriptional elongation in cancer cells*
**作者**：Wang Y, et al.
**摘要**：通过重组COBRA1蛋白体外实验，证实其通过抑制RNA聚合酶II的转录延伸活性调控致癌基因表达，与乳腺癌细胞增殖相关。
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**备注**：以上文献信息为示例性质，具体内容建议通过PubMed或Web of Science以关键词“COBRA1 recombinant human protein”检索最新研究。实际文献可能侧重不同领域(如神经发育、癌症机制等)，需根据研究背景筛选。
The human COBRA1 (co-factor of BRCA1) protein, also known as NELF-B, is a critical component of the negative elongation factor (NELF) complex, which regulates RNA polymerase II pausing during transcription. Initially identified as a BRCA1-interacting protein, COBRA1 plays dual roles in transcriptional regulation and genome maintenance. It is involved in modulating chromatin structure, DNA repair, and maintaining genomic stability, particularly in response to DNA damage. Structurally, COBRA1 contains a GATA-like zinc finger domain and facilitates NELF binding to nascent RNA, ensuring proper transcriptional elongation control.
Recombinant human COBRA1 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its recombinant form enables detailed biochemical analyses, including protein-protein/DNA interaction assays, chromatin remodeling studies, and investigations into transcriptional dysregulation in diseases. Research highlights COBRA1's involvement in cancer biology, as altered expression correlates with tumor progression and chemoresistance. Additionally, COBRA1 interacts with pathways critical for stem cell pluripotency and cellular differentiation, making it a focus in developmental biology. Its recombinant production supports therapeutic exploration, such as targeting transcription-coupled DNA repair in cancers.
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