| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COCH |
| Uniprot No | O43405 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 氨基酸序列 | MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAAK |
| 分子量 | 79.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COCH蛋白的3篇模拟文献示例(仅供参考，非真实文献)：
1. **《Expression and structural analysis of recombinant human COCH protein in Escherichia coli》**
*作者：Zhang L, et al.*
**摘要**：本研究成功在大肠杆菌中表达了重组人COCH蛋白，并通过X射线晶体学解析了其β-折叠富集结构，证实其具备类似细胞外基质的相互作用特性。
2. **《Functional characterization of COCH in Drosophila model reveals its role in extracellular matrix organization》**
*作者：Wang Y, et al.*
**摘要**：通过果蝇模型表达人源重组COCH蛋白，发现其与Perlecan蛋白互作，调控内耳基膜结构的稳定性，为遗传性耳聋机制提供新见解。
3. **《COCH mutations alter the glycosylation pattern of recombinant protein in HEK293 cells》**
*作者：Smith J, et al.*
**摘要**：在HEK293细胞中表达野生型和突变型重组COCH蛋白，发现DFNA9相关突变导致糖基化异常，影响其在内耳中的细胞粘附功能。
**注**：以上文献为基于COCH蛋白研究领域的常见方向构造的模拟案例，实际文献需通过PubMed或Google Scholar以关键词“recombinant human COCH protein”检索获取。
Recombinant human COCH protein, derived from the COCH gene (Coagulation Factor C Homology), is a subject of interest in auditory and vestibular research. The native COCH protein, primarily expressed in the inner ear's cochlea and vestibule, plays a critical role in maintaining extracellular matrix integrity and cellular homeostasis. Mutations in the COCH gene are linked to DFNA9. an autosomal dominant form of non-syndromic hearing loss and vestibular dysfunction, characterized by progressive degeneration of inner ear structures. This association underscores COCH's importance in auditory-vestibular physiology.
Recombinant COCH is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for structural and functional studies. Its recombinant form enables researchers to investigate COCH's molecular interactions, including binding partners like collagens and proteoglycans, without the complexity of isolating it from native tissues. Studies focus on its LIM2 and vWFA domains, implicated in protein-protein interactions and matrix organization. Recombinant COCH also serves as a tool to model DFNA9-related pathogenic mechanisms, test therapeutic strategies (e.g., gene therapy), and explore its potential as a biomarker for inner ear disorders. Current challenges include elucidating its precise mechanistic roles and developing targeted interventions for COCH-related hearing loss.
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