| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COA5 |
| Uniprot No | Q86WW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-74aa |
| 氨基酸序列 | MPKYYEDKPQGGACAGLKEDLGACLLQSDCVVQEGKSPRQCLKEGYCNSLKYAFFECKRSVLDNRARFRGRKGY |
| 分子量 | 8.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人COA5蛋白相关的3篇文献示例(文献为虚拟构造，仅供参考)：
1. **《COA5 mutations leading to impaired cytochrome c oxidase assembly cause cardiomyopathy》**
- 作者：Huigsloot M, et al.
- 摘要：研究揭示了COA5基因突变通过破坏细胞色素c氧化酶(COX)的组装导致线粒体功能缺陷，并与婴儿期心肌病相关。重组人COA5蛋白的体外功能分析表明其参与COX亚基的稳定性调控。
2. **《Recombinant expression and purification of human COA5 protein for functional studies》**
- 作者：Zhang Y, et al.
- 摘要：首次报道了重组人COA5蛋白在大肠杆菌中的可溶性表达及纯化方法，并通过质谱和圆二色谱验证其结构完整性，为后续功能研究提供基础。
3. **《COA5 interacts with mitochondrial ribosomes and supports mitochondrial translation》**
- 作者：Wang L, et al.
- 摘要：通过免疫共沉淀和蛋白质组学技术，发现重组COA5蛋白与线粒体核糖体相互作用，调控线粒体mRNA的翻译效率，进而影响氧化磷酸化系统的生物合成。
4. **《Structural insights into the role of COA5 in mitochondrial respiratory chain assembly》**
- 作者：Chen X, et al.
- 摘要：利用X射线晶体学解析重组COA5蛋白的三维结构，揭示其通过特定结构域与COX亚基结合，为理解线粒体呼吸链组装机制提供分子基础。
(注：上述文献为示例性内容，实际研究需以真实数据库检索结果为准。)
Recombinant human COA5 (Cytochrome c Oxidase Assembly Factor 5) protein is a mitochondria-targeted chaperone critical for the biogenesis of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain. COA5 facilitates the assembly of COX subunits and incorporation of heme and copper cofactors into the enzyme complex, which is essential for aerobic ATP production. Mutations in the COA5 gene are linked to mitochondrial disorders, particularly early-onset cardiomyopathy and COX deficiency, highlighting its role in maintaining cardiac and metabolic homeostasis. The protein contains conserved twin CX9C motifs, enabling its interaction with mitochondrial translocases during import. Recombinant COA5 is typically expressed in bacterial or mammalian systems (e.g., E. coli, HEK293) for functional studies, enabling investigation of pathogenic variants, protein-protein interactions, and COX assembly mechanisms. Its applications extend to modeling mitochondrial diseases, drug screening for COX-related pathologies, and exploring therapeutic interventions like gene therapy. Studies using recombinant COA5 have also provided insights into mitochondrial respiratory chain dynamics, stress responses, and the intersection of energy metabolism with cell survival pathways.
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