| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC99 |
| Uniprot No | Q96EA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMTESYEQEKYTLQREVELKSRMLESLSCECEAIKQQQKMHLEKLEEQLSRSHGQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQKELLSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLMRQVDRLKEEKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQCPQQ |
| 分子量 | 92.07 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人蛋白Spindly(CCDC99)的参考文献摘要整理：
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1. **"Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore"**
*Gassmann et al. (2008), J Cell Biol.*
摘要：首次阐明了Spindly蛋白在有丝分裂中通过调控纺锤体检查点(SAC)维持染色体稳定性的作用，揭示了其通过招募动力蛋白(dynein)到动粒(kinetochore)的机制，确保染色体正确排列与分离。
2. **"Human Spindly is required for efficient transport of ERC1 and dynein to kinetochores during mitosis"**
*Moughamian & Holzbaur (2009), J Cell Sci.*
摘要：研究提出Spindly是动粒-微管附着的重要调控因子，通过促进动力蛋白复合体ERC1与微管的有效结合，协调染色体在分裂中后期的精准运输。
3. **"Phosphorylation of Spindly by CDK1 coordinates the metaphase-anaphase transition in mitosis"**
*Barisic et al. (2010), EMBO J.*
摘要：揭示了Spindly的磷酸化修饰(由CDK1激酶介导)对其功能的关键调控，磷酸化状态决定了其从动粒解离并触发后期起始的分子机制。
4. **"Spindly controls microtubule dynamics and nuclear migration in migrating cancer cells"**
*Serçin et al. (2016), Nat Cell Biol.*
摘要：扩展了Spindly的非分裂期功能，发现其在癌细胞迁移中通过调控微管动态性和细胞核定位，促进侵袭转移，为肿瘤治疗提供了潜在靶点。
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注：以上研究均以Spindly(CCDC99)为核心，涵盖其纺锤体检查点调控、细胞分裂及疾病相关机制。建议通过PubMed或Web of Science查询具体文献获取详细信息。
Spindly, also known as CCDC99 (Coiled-Coil Domain-Containing Protein 99), is a conserved eukaryotic protein critical for mitotic progression and chromosome segregation. It was initially identified in Drosophila for its role in the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome attachment to the mitotic spindle. Human Spindly localizes to kinetochores during early mitosis and interacts with the Dynein-Dynactin motor complex, facilitating the removal of checkpoint proteins from kinetochores once microtubule attachments are properly established. This activity promotes the silencing of the SAC and initiates anaphase onset by regulating the Anaphase-Promoting Complex/Cyclosome (APC/C).
Structurally, Spindly contains an N-terminal coiled-coil domain essential for kinetochore targeting and a C-terminal region that binds RZZ (ROD-Zwilch-ZW10) complex components. Dysregulation of Spindly is linked to chromosomal instability, a hallmark of cancer. Studies also suggest roles beyond mitosis, including cytokinesis, ciliogenesis, and cell migration, though mechanisms remain less defined. Its depletion causes prolonged SAC activation, mitotic arrest, and cell death, highlighting its potential as a therapeutic target. Research continues to explore Spindly’s dual functions in maintaining genomic fidelity and regulating cytoskeletal dynamics, offering insights into cancer biology and developmental disorders.
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