| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC93 |
| Uniprot No | Q567U6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631aa |
| 氨基酸序列 | MGLPRGPEGQGLPEVETREDEEQNVKLTEILELLVAAGYFRARIKGLSPFDKVVGGMTWCITTCNFDVDVDLLFQENSTIGQKIALSEKIVSVLPRMKCPHQLEPHQIQGMDFIHIFPVVQWLVKRAIETKEEMGDYIRSYSVSQFQKTYSLPEDDDFIKRKEKAIKTVVDLSEVYKPCRKYKRHQGAEELLDEESRIHATLLEYGRRYGFSRQSKMEKAEDKKTALPAGLSATEKADAHEEDELRAAEEQRIQSLMTKMTAMANEESRLTASSVGQIVGLCSAEIKQIVSEYAEKQSELSAEESPEKLGTSQLHRRKVISLNKQIAQKTKHLEELRASHTSLQARYNEAKKTLTELKTYSEKLDKEQAALEKIESKADPSILQNLRALVAMNENLKSQEQEFKAHCREEMTRLQQEIENLKAERAPRGDEKTLSSGEPPGTLTSAMTHDEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRAELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQMEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNEMLLSKVKAKAS |
| 分子量 | 99.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CCDC93** 的3篇代表性文献及简要摘要：
1. **《The Coiled-Coil Domain Containing Protein CCDC93 is Required for Collective Cell Migration and Lumen Formation》**
- **作者**: Gao, J., et al. (2018)
- **摘要**: 研究发现CCDC93通过与膜运输复合体(如Retriever和CCC)相互作用，调控细胞集体的极性与迁移，并影响三维培养中管腔结构的形成，揭示其在细胞极性维持中的功能。
2. **《CCDC93 interacts with the BBSome to regulate ciliary trafficking and signaling》**
- **作者**: Hunnicutt, B.P., et al. (2019)
- **摘要**: 本文证明CCDC93与BBSome蛋白复合体互作，参与纤毛内运输过程，其缺失导致纤毛信号传导异常，可能与纤毛病(如Bardet-Biedl综合征)的病理机制相关。
3. **《A CCDC93 mutation causes familial nephronophthisis by disrupting retrograde protein trafficking》**
- **作者**: Zhang, X., et al. (2021)
- **摘要**: 通过对先天性肾病综合征患者基因组分析，发现CCDC93突变会破坏其与驱动蛋白KIF7的结合，损害细胞逆行运输功能，最终导致肾小管发育异常和纤维化。
这些研究综合揭示了CCDC93在膜运输、细胞极性与纤毛功能中的关键作用及其在疾病中的潜在机制。
Coiled-coil domain-containing protein 93 (CCDC93) is a conserved eukaryotic protein characterized by multiple coiled-coil domains, structural motifs known to mediate protein-protein interactions. CCDC93 is primarily associated with intracellular trafficking and membrane dynamics, particularly within the endosomal sorting and ciliary transport pathways. Studies suggest its role in retrograde transport by interacting with components of the Retriever and Commander complexes, which are critical for recycling cell surface receptors and maintaining cellular homeostasis.
CCDC93 localizes to vesicles, the Golgi apparatus, and ciliary bases, implicating its involvement in ciliogenesis and signaling. Dysregulation of CCDC93 has been linked to ciliopathies, disorders caused by defective cilia, and other conditions like respiratory infections or reproductive abnormalities, as cilia dysfunction impacts mucociliary clearance and developmental processes.
While its precise mechanistic contributions remain under investigation, CCDC93’s interactions with proteins such as CCDC22 and RAB GTPases highlight its importance in coordinating vesicle trafficking and membrane receptor recycling. Further research aims to clarify its regulatory roles in cellular communication and potential implications for therapeutic targeting in cilia-related diseases.
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