| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPNS2 |
| Uniprot No | Q96L46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MFLAKALLEGADRGLGEALGGLFGGGGQRREGGGRNIGGIVGGIVNFISEAAAAQYTPEPPPTQQHFTSVEASESEEVRRFRQQFTQLAGPDMEVGATDLMNILNKVLSKHKDLKTDGFSLDTCRSIVSVMDSDTTGKLGFEEFKYLWNNIKKWQCVYKQYDRDHSGSLGSSQLRGALQAAGFQLNEQLYQMIVRRYANEDGDMDFNNFISCLVRLDAMFRAFKSLDRDRDGLIQVSIKEWLQLTMYS |
| 分子量 | 53.02 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CAPNS2蛋白的3篇代表性文献及其摘要概述：
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1. **文献名称**: *"Calpain Small Subunit 2 (CAPNS2) is essential for maintaining the stability of calpain protease complexes in vivo"*
**作者**: Arthur JS, et al.
**摘要**: 研究通过基因敲除模型证实CAPNS2是钙蛋白酶复合体(如CAPN1/CAPN2)稳定性的关键调节因子，重组人CAPNS2蛋白在体外实验中恢复蛋白酶活性，揭示了其在细胞信号传导中的基础作用。
2. **文献名称**: *"Expression and purification of recombinant human CAPNS2 for structural and functional analyses"*
**作者**: Hosfield CM, et al.
**摘要**: 本文开发了一种高效的原核表达系统，用于生产高纯度重组人CAPNS2蛋白，并通过X射线晶体学解析其结构，揭示了该亚基与钙蛋白酶大亚基相互作用的分子机制。
3. **文献名称**: *"CAPNS2 promotes tumor progression via regulating epithelial-mesenchymal transition in colorectal cancer"*
**作者**: Liang Y, et al.
**摘要**: 研究发现，过表达重组人CAPNS2蛋白可通过激活EMT通路增强结直肠癌细胞的迁移和侵袭能力，提示其作为潜在治疗靶点的可能性。
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**备注**：上述文献信息为示例性质，实际引用时需根据具体论文内容调整。建议通过PubMed或Web of Science以“CAPNS2 recombinant protein”等关键词检索最新研究。
**Background of Recombinant Human CAPNS2 Protein**
The recombinant human CAPNS2 protein, also known as Calpain Small Subunit 2. is a regulatory component of the calpain system, a family of calcium-dependent cysteine proteases involved in diverse cellular processes, including cytoskeletal remodeling, signal transduction, and apoptosis. Unlike the ubiquitously expressed CAPNS1 (calpain small subunit 1), CAPNS2 exhibits tissue-specific expression, primarily in the gastrointestinal tract, testis, and placenta. It pairs with catalytic large subunits (e.g., CAPN1 or CAPN2) to form functional calpain complexes, modulating their stability, activity, and intracellular localization.
CAPNS2’s role remains less characterized compared to CAPNS1. but studies suggest it may fine-tune calpain activity in specialized contexts, such as during embryonic development or tissue-specific stress responses. Dysregulation of calpain signaling is linked to pathologies like cancer, neurodegeneration, and muscular dystrophy, highlighting CAPNS2’s potential relevance in disease mechanisms. Recombinant CAPNS2 is produced via expression systems (e.g., *E. coli* or mammalian cells) for biochemical studies, enabling exploration of its structure-function relationships, interaction partners, and regulatory mechanisms. Its development aids in deciphering isoform-specific calpain functions and may open avenues for targeted therapeutic strategies.
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