| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALML6 |
| Uniprot No | Q8TD86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MGLQQEISLQPWCHHPAESCQTTTDMTERLSAEQIKEYKGVFEMFDEEGNGEVKTGELEWLMSLLGINPTKSELASMAKDVDRDNKGFFNCDGFLALMGVYHEKAQNQESELRAAFRVFDKEGKGYIDWNTLKYVLMNAGEPLNEVEAEQMMKEADKDGDRTIDYEEFVAMMTGESFKLIQ |
| 分子量 | 46.31 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人CALML6蛋白的参考文献示例(部分内容为假设性概括，实际引用需核实原文)：
1. **《CALML6 regulates invasion and angiogenesis in breast cancer through calcium signaling》**
- **作者**: Zhang Y, et al.
- **摘要**: 本研究首次表达了重组人CALML6蛋白，并发现其通过钙离子信号通路增强乳腺癌细胞的侵袭能力，同时促进肿瘤血管生成。实验表明，CALML6通过与VEGF受体互作调节下游通路。
2. **《Structural and functional characterization of recombinant human CALML6》**
- **作者**: Lee S, et al.
- **摘要**: 该研究利用大肠杆菌系统成功表达并纯化了重组CALML6蛋白，通过X射线晶体学解析其三维结构，揭示了其钙离子结合域的独特构象，并证明其钙依赖性调控靶蛋白的能力。
3. **《CALML6 as a novel biomarker for early-stage lung adenocarcinoma》**
- **作者**: Wang X, et al.
- **摘要**: 利用重组CALML6蛋白制备抗体，发现其在早期肺腺癌患者血清中显著高表达。功能实验显示CALML6通过激活MAPK通路促进癌细胞增殖，提示其作为诊断标志物的潜力。
4. **《Interactome profiling of CALML6 identifies binding partners in epidermal differentiation》**
- **作者**: Gupta R, et al.
- **摘要**: 通过重组CALML6蛋白进行免疫共沉淀-质谱分析，鉴定出其在表皮分化中与角蛋白及钙调磷酸酶互作，调控角质形成细胞的分化进程，为皮肤病机制研究提供新方向。
**提示**：以上文献标题及内容为模拟概括，实际研究可能存在差异。建议通过PubMed或Web of Science以“CALML6 recombinant”为关键词检索最新论文，并核对作者与摘要准确性。
**Background of Recombinant Human CALML6 Protein**
CALML6 (Calmodulin-Like Protein 6) belongs to the calmodulin family, a group of calcium-sensing proteins crucial for mediating calcium-dependent signaling pathways. Unlike canonical calmodulin, CALML6 is classified as a "calmodulin-like" protein due to structural variations, including a distinct EF-hand domain organization that influences its calcium-binding affinity and target interactions. The human CALML6 gene is located on chromosome 1 and encodes a small, highly conserved protein predominantly expressed in epithelial tissues, including the skin, esophagus, and reproductive organs.
Recombinant CALML6 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties and physiological roles. Studies suggest CALML6 regulates cell differentiation, proliferation, and cytoskeletal dynamics, potentially acting as a tumor suppressor in certain cancers by modulating pathways like Wnt/β-catenin. Its involvement in keratinocyte differentiation highlights relevance to skin barrier function and wound healing.
Research on recombinant CALML6 also explores its diagnostic or therapeutic potential, particularly in epithelial cancers or inflammatory disorders. Structural analyses using recombinant protein have clarified its calcium-dependent conformational changes, aiding mechanistic insights into its interaction partners. However, the full scope of CALML6's biological functions remains under investigation, necessitating further studies to unravel its roles in health and disease.
×
