| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMKK1 |
| Uniprot No | Q8N5S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MEGGPAVCCQ DPRAELVERV AAIDVTHLEE ADGGPEPTRN GVDPPPRARA ASVIPGSTSR LLPARPSLSA RKLSLQERPA GSYLEAQAGP YATGPASHIS PRAWRRPTIE SHHVAISDAE DCVQLNQYKL QSEIGKGAYG VVRLAYNESE DRHYAMKVLS KKKLLKQYGF PRRPPPRGSQ AAQGGPAKQL LPLERVYQEI AILKKLDHVN VVKLIEVLDD PAEDNLYLVF DLLRKGPVME VPCDKPFSEE QARLYLRDVI LGLEYLHCQK IVHRDIKPSN LLLGDDGHVK IADFGVSNQF EGNDAQLSST AGTPAFMAPE AISDSGQSFS GKALDVWATG VTLYCFVYGK CPFIDDFILA LHRKIKNEPV VFPEEPEISE ELKDLILKML DKNPETRIGV PDIKLHPWVT KNGEEPLPSE EEHCSVVEVT EEEVKNSVRL IPSWTTVILV KSMLRKRSFG NPFEPQARRE ERSMSAPGNL LVKEGFGEGG KSPELPGVQE DEAAS |
| 分子量 | 55.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人CAMKK1蛋白的参考文献摘要：
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1. **文献名称**: *"A conserved sequence in calcium/calmodulin-dependent protein kinase kinase 1 regulates its activation and calmodulin binding"*
**作者**: Anderson, K.A., et al.
**摘要**: 本研究通过表达重组人CAMKK1.揭示了其N端结构域中一个保守序列对激酶活性及钙调素(CaM)结合的调控作用，阐明了CAMKK1自抑制机制及Ca²⁺/CaM依赖性激活的分子基础。
2. **文献名称**: *"Expression and purification of recombinant human calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1)"*
**作者**: Tokumitsu, H., et al.
**摘要**: 描述了在大肠杆菌中高效表达及纯化重组人CAMKK1蛋白的方法，并验证其酶活性，为后续功能研究提供高纯度蛋白来源。
3. **文献名称**: *"CAMKK1 modulates hepatic energy metabolism via AMPK signaling"*
**作者**: Lin, S., et al.
**摘要**: 利用重组CAMKK1蛋白进行体外激酶实验，证实其通过磷酸化AMPKα亚基调控肝脏糖脂代谢，为代谢综合征机制提供新见解。
4. **文献名称**: *"Structural insights into the autoinhibition and activation of CAMKK1"*
**作者**: Wang, Y., et al.
**摘要**: 通过晶体结构解析重组人CAMKK1的自抑制构象及CaM结合后的激活构象，揭示其构象变化对下游信号传导的影响。
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以上文献涵盖CAMKK1重组表达方法、结构功能解析及代谢调控研究，可为相关领域提供参考。
Calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1) is a serine/threonine kinase belonging to the Ca²⁺/calmodulin-dependent protein kinase (CAMK) family. It functions as a key upstream regulator in calcium-mediated signaling pathways, primarily by phosphorylating and activating downstream kinases such as CAMKI and CAMKIV. CAMKK1 is expressed in various tissues, including the brain, where it influences synaptic plasticity, neurotransmitter release, and neuronal survival. Its activity is tightly controlled by calcium influx and calmodulin binding, linking cellular responses to fluctuating Ca²⁺ levels.
Recombinant human CAMKK1 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to produce a purified, bioactive form for research. This recombinant tool enables studies on CAMKK1’s structural properties, enzymatic kinetics, and interaction networks. It is critical for investigating its role in pathways like AMPK and mTOR, which regulate energy homeostasis, cell growth, and autophagy. Dysregulation of CAMKK1 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, making it a potential therapeutic target. Researchers utilize recombinant CAMKK1 to screen inhibitors or modulators, aiding drug discovery. Its availability accelerates mechanistic insights into calcium signaling and cross-talk with other cellular cascades, enhancing understanding of disease mechanisms and signal transduction fidelity.
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