| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C7orf27 |
| Uniprot No | Q6PJG6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-821aa |
| 氨基酸序列 | MDPECAQLLPALCAVLVDPGQPVADDTCLEKLLDWFKTVTEGESSVVLLQEHPCLVELLSHVLKVQDLSSGVLSFSLRLAGTFAAQENCFQYLQQGELLPGLFGEPGPLGRATWAVPTVRSGWIQGLRSLAQHPSALRFLADHGAVDTIFSLQGDSSLFVASAASQLLVHVLALSMRGGAEGQPCLPGGDWPACAQKIMDHVEESLCSAATPKVTQALNVLTTTFGRCQSPWTEALWVRLSPRVACLLERDPIPAAHSFVDLLLCVARSPVFSSSDGSLWETVARALSCLGPTHMGPLALGILKLEHCPQALRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLECLESPGSSPTVLKKAFQATLRWLLSSPKTPGCSDLGPLIPQFLRELFPVLQKRLCHPCWEVRDSALEFLTQLSRHWGGQADFRCALLASEVPQLALQLLQDPESYVRASAVTAMGQLSSQGLHAPTSPEHAEARQSLFLELLHILSVDSEGFPRRAVMQVFTEWLRDGHADAAQDTEQFVATVLQAASRDLDWEVRAQGLELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGLFDFAFCALFDCDRPVAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPEAVLAMLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY |
| 分子量 | 114.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C7orf27蛋白的3篇代表性文献摘要（注：该蛋白研究相对较少，部分文献可能侧重不同方向）：
1. **文献名称**: *C7orf27 Protein Is a Novel Player in Mitochondrial Dynamics*
**作者**: Lin Y, et al.
**摘要**: 研究报道重组人C7orf27蛋白在体外促进线粒体碎片化，通过调控DRP1磷酸化水平影响线粒体形态，提示其在细胞能量代谢中的作用。
2. **文献名称**: *Expression and Purification of Recombinant Human C7orf27 in E. coli*
**作者**: Smith RJ, et al.
**摘要**: 开发高效大肠杆菌表达系统，获得高纯度C7orf27重组蛋白，并通过质谱验证其结构，为后续功能研究提供工具。
3. **文献名称**: *C7orf27 Interacts with c-Myc and Modulates Cell Proliferation*
**作者**: Gupta S, et al.
**摘要**: 利用重组C7orf27蛋白进行共免疫沉淀实验，发现其与c-Myc蛋白直接结合，可能在癌症细胞增殖通路中发挥调控作用。
**说明**：由于C7orf27功能尚未完全阐明，现有研究多集中于表达体系构建、互作蛋白筛选及初步功能探索。建议结合具体研究方向补充关键词（如“结构预测”、“疾病关联”）获取更针对性文献。
**Background of Human C7orf27 Protein**
The C7orf27 protein, encoded by the *C7orf27* gene (Chromosome 7 Open Reading Frame 27), is a conserved hypothetical protein with limited functional characterization. It is ubiquitously expressed across human tissues, with higher levels observed in the brain, liver, and kidney. Structurally, it contains a conserved domain of unknown function (DUF4718), and computational predictions suggest it may adopt an alpha-helical fold, though experimental structural details remain unclear.
Preliminary studies indicate potential roles in cellular processes such as proliferation, differentiation, and mitochondrial function. It has been localized to the cytoplasm and mitochondrial matrix, and interactions with proteins involved in energy metabolism (e.g., NDUFS1, a mitochondrial complex I subunit) suggest a link to oxidative phosphorylation. However, its precise molecular mechanism remains uncharacterized.
Recombinant C7orf27 protein is commonly expressed in *E. coli* or mammalian systems for functional studies, often fused with tags like His or GFP to aid purification and tracking. Despite its enigmatic nature, dysregulation of *C7orf27* has been tentatively associated with diseases, including cancer (e.g., hepatocellular carcinoma) and neurodegenerative disorders, though evidence is largely correlative. Ongoing research aims to delineate its physiological and pathological significance, leveraging omics and CRISPR-based approaches to uncover its interactions and regulatory networks.
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