| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C6orf81 |
| Uniprot No | Q5T9G4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MGKSIPQYLGQLDIRKSVVSLATGAGAIYLLYKAIKAGIKCKPPLCSNSPICIARECPGPGERALPQEAPAPEASAVGGPKGLAVERERHGRDSGELRRLLNSLECKQDEYAKSMILHSITRCVYLLEAEASACTTDDIVLLGYMLDDKDNSVKTQALNTLKAFSGIRKFRLKIQEHSIKVLELISTIWDTELHIAGLRLLNNLPLPDYVHPQLRRVMPALMEILQSDYILAQVQAVRLLSYLAQKNDLLYDILNCQVHSNFLNLFQPTQSGSLLYEVLVFAERLSEGRNAPHYHVVKWHYNEQSLHESLFGEESRLADRLLALVIHPEEDVQIQACKVIVSLQYPQDLRARPSSCQPSRSYFKNTE |
| 分子量 | 67.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C6orf81蛋白的模拟参考文献示例（需注意实际文献可能有限，建议通过学术数据库验证）：
1. **文献名称**: *Functional Characterization of Recombinant Human C6orf81 Protein in Neuronal Development*
**作者**: Lee et al. (2020)
**摘要**: 研究了重组人C6orf81蛋白在体外神经元分化中的作用，发现其通过调控Wnt/β-catenin信号通路促进轴突生长，暗示其在神经发育疾病中的潜在角色。
2. **文献名称**: *C6orf81 Interacts with Mitochondrial Proteins: Implications for Energy Metabolism*
**作者**: Zhang & Patel (2018)
**摘要**: 利用重组C6orf81蛋白进行酵母双杂交筛选，鉴定其与线粒体复合体IV亚基的相互作用，提示该蛋白可能参与细胞能量代谢调节。
3. **文献名称**: *Structural Analysis of Recombinant C6orf81 Reveals a Novel Zinc-Binding Domain*
**作者**: Müller et al. (2019)
**摘要**: 通过X射线晶体学解析C6orf81蛋白的晶体结构，发现其C端含有一个新型锌结合基序，为理解其分子功能提供了结构基础。
4. **文献名称**: *C6orf81 Knockdown and Overexpression Models Link the Protein to Autophagy Regulation*
**作者**: Kimura et al. (2021)
**摘要**: 在细胞模型中验证重组C6orf81蛋白的表达水平对自噬活性的影响，发现其可能通过mTOR通路参与应激条件下的细胞存活机制。
**提示**：C6orf81研究尚较新兴，建议结合基因别名（如HSPC306）或功能关键词（如染色体6开放读码框81）扩展检索，并关注近年的功能基因组学研究。
**Background of Recombinant Human C6orf81 Protein**
The C6orf81 (chromosome 6 open reading frame 81) gene, located on human chromosome 6, encodes a protein whose biological function remains largely uncharacterized. Current genomic annotations suggest it is evolutionarily conserved across vertebrates, hinting at potential roles in fundamental cellular processes. Structural predictions indicate that the C6orf81 protein may contain transmembrane domains or intrinsically disordered regions, though experimental validation is lacking. Interest in recombinant C6orf81 stems from its association with diseases in genome-wide studies, including links to cancer progression and neurodegenerative disorders, though mechanistic insights are sparse.
Recombinant production of human C6orf81 in heterologous systems (e.g., *E. coli* or mammalian cells) enables biochemical and functional studies. Initial research focuses on identifying interaction partners, post-translational modifications, and subcellular localization to delineate its role in signaling, metabolism, or structural regulation. Challenges include low endogenous expression levels and absence of specific antibodies, complicating *in vivo* analyses. Advances in proteomics and CRISPR-based screening may accelerate its functional elucidation, with potential implications for understanding disease pathways or therapeutic targeting. Despite its obscurity, C6orf81 represents an underexplored protein warranting systematic investigation.
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