| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf45 |
| Uniprot No | Q8N5I9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185aa |
| 氨基酸序列 | MEVHGKPKAS PSCSSPTRDS SGVPVSKELL TAGSDGRGGI WDRLLINSQP KSRKTSTLQT VRIERSPLLD QVQTFLPQMA RANEKLRKEM AAAPPGRFNI ENIDGPHSKV IQMDVALFEM NQSDSKEVDS SEESSQDSSE NSSESEDEDD SIPSEVTIDN IKLPNSEGGK GKIEVLDSPA SKKKK |
| 分子量 | 20 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C12orf45蛋白的3条参考文献示例(注：以下为虚构示例，实际文献需通过数据库检索验证)：
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1. **文献名称**: *Recombinant Expression and Functional Analysis of C12orf45 in Cancer Cell Lines*
**作者**: Zhang, L. et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组C12orf45蛋白，并验证其通过调控MAPK信号通路促进乳腺癌细胞侵袭和转移的分子机制。
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2. **文献名称**: *C12orf45 Interacts with Mitochondrial Proteins: Implications for Apoptosis Regulation*
**作者**: Kumar, R. & Lee, S.
**摘要**: 通过免疫共沉淀实验发现，C12orf45与Bcl-2家族蛋白存在相互作用，提示其在细胞凋亡调控中可能发挥关键作用。
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3. **文献名称**: *Structural Characterization of Human C12orf45 Using Cryo-EM*
**作者**: Tanaka, M. et al.
**摘要**: 首次解析了重组C12orf45蛋白的冷冻电镜结构，揭示其独特的α-螺旋结构域可能参与DNA损伤修复过程。
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**提示**：实际研究中，C12orf45相关文献较少，建议扩展检索策略(如关联基因别名或表型关键词)。可通过PubMed等平台以“C12orf45 recombinant protein”或“C12orf45 function”为关键词查找真实文献。
**Background of Recombinant Human C12orf45 Protein**
The C12orf45 (Chromosome 12 Open Reading Frame 45) gene, also known as MINERVA, encodes a protein with unclear molecular functions, though preliminary studies suggest potential roles in cellular processes like autophagy, stress response, and cell proliferation. Located on human chromosome 12. this gene is evolutionarily conserved across vertebrates, hinting at biological significance. The endogenous C12orf45 protein is predicted to contain disordered regions and lacks well-defined structural domains, complicating functional characterization.
Interest in recombinant human C12orf45 protein stems from its differential expression in cancers, including hepatocellular carcinoma and glioblastoma, where it may act as an oncogenic modifier or biomarker. Recombinant versions are typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. These tools enable studies on its interactome, post-translational modifications, and mechanisms in disease models.
Despite progress, C12orf45 remains understudied, with its precise physiological and pathological roles unresolved. Ongoing research focuses on elucidating its involvement in signaling pathways and therapeutic potential. The development of recombinant C12orf45 protein is critical for advancing structural, biophysical, and functional analyses to uncover its biological relevance.
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