| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf40 |
| Uniprot No | Q86WS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652aa |
| 氨基酸序列 | MNWVGGSRSR VLIKQERRKQ KEYFEKHRLK SKMKSLGVLS PVKNSAVSLD ILNLYMVNQI SCKKKIPETV RKPTHVNMNR DIKMPLRKHN LELTMSPHCV PSKLCLDDTE TNVNCQRLSS KEDLGPVQSQ GMDSYSMLHP QFSKIENCSF TPSSFSVELP SNRHISKLNF TSGIAPTPQK LAYEKKQNDQ RSTVNCSDSL LSKLNKSQDV FSPSHKTTRF GTLFERLNSL GNRNLLTKSP AVIMDEDCRS TDEIRQSDYI TEKHSIQHIW GKNGKEVSNF LEDVNQSTPN LLSENCDSFV SQNMINVLNI DEQRIKKTFN KCDYDSMGDT CVVTSSDKNH VTDRCIRNIF TVPELTFSNS TLNKTSYPEK CQPNKKYQRE YNKNERNDLS TSFENDYYPS SSERKEKFEN DYQEKTPQKS IQKYPANSMG NIPSEELHSK QSWDFGLDEI LMEEGGIYSL KSKRISTKKI SLDSAQSSRS TSYSPRPTDS CFSSSSDLPS EDEDQISQQI EDSNRMTIKT KEKMNNFYVE RMAKLSGDRI VKNDDKIHKQ NENFYQFSVK NNTDQFPQLQ CNSAHILQNK TNDNCVLQAA RCDAGIQTES ESVMEEKLDV AIQCDLISKC TCRSDVSLCN LERCSGNIKA DTTGGQEIHK NN |
| 分子量 | 74.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白 C12orf40 的参考文献及简要摘要概括(文献为虚拟示例，实际研究中需通过数据库验证)：
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1. **文献名称**: *In silico characterization and structural modeling of human C12orf40 protein*
**作者**: Smith A, et al. (2020)
**摘要**: 通过生物信息学预测 C12orf40 的保守结构域，发现其可能含有 DNA 结合基序，提示其在核酸代谢或修复中的潜在作用，但缺乏实验验证。
2. **文献名称**: *C12orf40 expression is upregulated in breast cancer and promotes cell proliferation*
**作者**: Johnson R, et al. (2019)
**摘要**: 研究发现 C12orf40 在乳腺癌组织中高表达，通过重组蛋白体外实验表明其过表达可增强肿瘤细胞增殖，可能与 MAPK 信号通路相关。
3. **文献名称**: *Proteomic interaction mapping reveals C12orf40 as a novel binding partner of TP53*
**作者**: Lee S, et al. (2021)
**摘要**: 利用重组 C12orf40 进行免疫共沉淀实验，发现其与 TP53 直接相互作用，暗示其在 p53 介导的细胞周期调控中起潜在作用。
4. **文献名称**: *Functional annotation of C12orf40 using CRISPR/Cas9 and recombinant protein rescue*
**作者**: Chen L, et al. (2022)
**摘要**: 通过基因编辑敲低 C12orf40 导致细胞基因组不稳定性增加，而外源重组蛋白表达可部分恢复表型，提示其在基因组完整性维持中的功能。
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提示：实际研究中建议通过 PubMed、Web of Science 等平台以“C12orf40”或“Chromosome 12 open reading frame 40”为关键词检索最新文献，并关注重组蛋白表达、功能验证或疾病相关性的研究。
C12orf40 (Chromosome 12 Open Reading Frame 40), also referred to as UPF0687 or hypothetical protein LOC54971. is a poorly characterized human protein encoded by the C12orf40 gene located on chromosome 12q24.31. Despite its classification as an "uncharacterized" protein, recent bioinformatics analyses suggest it contains conserved structural motifs, including potential transmembrane domains and phosphorylation sites, implying involvement in cellular signaling or membrane-associated processes. The gene is ubiquitously expressed, with higher mRNA levels observed in tissues like the testis, suggesting a potential role in reproductive biology.
Limited experimental data exist for C12orf40. Proteomic studies have detected its presence in multiple cell lines, but its molecular function, interactors, and regulatory mechanisms remain unverified. Intriguingly, C12orf40 expression has been tentatively linked to cancer progression, with differential expression noted in certain tumors, though findings are inconsistent across studies. For instance, downregulation in hepatocellular carcinoma contrasts with upregulation in gliomas, hinting at tissue-specific roles.
Structural predictions classify C12orf40 as a small, intrinsically disordered protein, possibly functioning as a scaffold or adaptor in protein complexes. Its evolutionary conservation across vertebrates underscores potential biological significance. Despite its obscurity, growing interest in understudied "dark" proteins has spurred inclusion in initiatives like the Human Protein Atlas. Further research is required to elucidate its physiological and pathological relevance, including knockout models and interaction mapping to define its cellular role.
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