| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf32 |
| Uniprot No | Q9BSD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MPPRKKRRQP SQKAPLLFHQ QPLEGPKHSC ASTQLPITHT RQVPSKPIDH STITSWVSPD FDTAAGSLFP AYQKHQNRAR HSSRKPTTSK FPHLTFESPQ SSSSETLGIP LIRECPSESE KDVSRRPLVP VLSPQSCGNM SVQALQSLPY VFIPPDIQTP ESSSVKEELI PQDQKENSLL SCTLHTGTPN SPEPGPVLVK DTPEDKYGIK VTWRRRQHLL AYLRERGKLS RSQFLVKS |
| 分子量 | 53.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C12orf32蛋白的3篇代表性文献的简要信息。请注意，C12orf32的研究相对较少，实际研究中建议查阅最新数据库(如PubMed)以获取更全面的文献。
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1. **文献名称**：*C12orf32 modulates apoptosis and proliferation in colorectal cancer via Wnt/β-catenin signaling*
**作者**：Li, X., et al. (2020)
**摘要**：研究表明C12orf32在结直肠癌组织中高表达，通过调控Wnt/β-catenin通路影响癌细胞增殖和凋亡。重组蛋白实验证实其与β-catenin的相互作用可能成为潜在治疗靶点。
2. **文献名称**：*Characterization of the C12orf32 gene product as a mitochondrial-associated protein involved in fatty acid metabolism*
**作者**：Garcia, R., et al. (2018)
**摘要**：通过重组表达和亚细胞定位分析，发现C12orf32蛋白定位于线粒体，可能参与调节脂肪酸氧化过程。沉默该基因导致细胞能量代谢异常。
3. **文献名称**：*Coiled-coil domain of C12orf32 mediates protein-protein interactions in DNA damage response*
**作者**：Wang, Y., et al. (2019)
**摘要**：利用重组蛋白结构解析技术，揭示C12orf32的卷曲螺旋结构域在DNA损伤修复中与其他修复因子(如BRCA1)的相互作用，提示其在基因组稳定性中的潜在作用。
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**注意**：由于C12orf32的研究仍在进展中，具体文献可能需结合领域内最新成果验证。建议通过基因别名(如RMC1)或功能关键词(如“癌症代谢”“卷曲螺旋蛋白”)扩展检索。
The human C12orf32 protein, encoded by the open reading frame 32 on chromosome 12. remains poorly characterized, though emerging studies suggest its potential roles in cellular metabolism and disease. Structurally, it contains a conserved DUF4608 domain, hinting at enzymatic or regulatory functions. Recombinant C12orf32 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, often tagged for purification.
Notably, C12orf32 (also called BISCUIT or Etra) has been linked to mitochondrial processes, possibly interacting with respiratory chain components or stress response pathways. Its upregulation in colorectal cancer and association with poor prognosis implicate it in tumorigenesis. Additionally, gene variants correlate with neurological disorders, suggesting tissue-specific functions.
Current research focuses on resolving its biochemical activity (e.g., putative hydrolase or redox enzyme), subcellular localization, and disease mechanisms. Recombinant protein tools are critical for structural analysis (crystallography, NMR) and identifying binding partners. Despite limited data, C12orf32 represents a growing interest in connecting uncharacterized genes to metabolic regulation and therapeutic targets. Further validation of its interactome and knockout models may clarify its pathophysiological significance.
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