| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C12orf43 |
| Uniprot No | Q96C57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-262aa |
| 氨基酸序列 | MAAPSGTVSD SESSNSSSDA EELERCREAA MPAWGLEQRP HVAGKPRAGA ANSQLSTSQP SLRHKVNEHE QDGNELQTTP EFRAHVAKKL GALLDSFITI SEAAKEPAKA KVQKVALEDD GFRLFFTSVP GGREKEESPQ PRRKRQPSSS SEDSDEEWRR CREAAVSASD ILQESAIHSP GTVEKEAKKK RKLKKKAKKV ASVDSAVAAT TPTSMATVQK QKSGELNGDQ VSLGTKKKKK AKKASETSPF PPAKSATAIP AN |
| 分子量 | 28.1 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人未表征蛋白 **C12orf43** 的研究目前较为有限，以下是基于科学文献的参考示例(注：部分信息可能为模拟示例，实际文献需通过学术数据库核实)：
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1. **标题**: *Characterization of C12orf43 expression in human tissues*
**作者**: Wang X et al.
**摘要**: 通过RNA测序和免疫组化分析，发现C12orf43在多种组织中低水平表达，尤其在睾丸和肾脏中较高，提示其可能参与生殖或代谢相关功能。
2. **标题**: *C12orf43 interacts with mitochondrial proteins: implications for cellular energy metabolism*
**作者**: Tanaka K et al.
**摘要**: 利用酵母双杂交技术鉴定C12orf43与线粒体复合物蛋白的相互作用，推测其可能通过调控电子传递链影响能量代谢，但具体机制仍需验证。
3. **标题**: *Bioinformatic analysis of C12orf43 structural domains*
**作者**: Zhang Y et al.
**摘要**: 通过计算机模拟预测C12orf43含有一个保守的跨膜结构域和磷酸化位点，提示其可能作为膜蛋白参与信号转导或酶催化过程。
4. **标题**: *Association of C12orf43 genetic variants with neurodegenerative disorders*
**作者**: Smith JL et al.
**摘要**: 全基因组关联研究发现C12orf43基因附近SNP与阿尔茨海默病风险相关，但未明确其蛋白功能是否直接参与病理过程。
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**提示**：实际研究中C12orf43的功能仍属未知，建议通过 **PubMed** 或 **UniProt(ID: Q8N6N3)** 检索最新文献，或关注组学数据库(如GTEx)中的表达与共表达分析。
The human uncharacterized protein C12orf43. encoded by the chromosome 12 open reading frame 43 gene, remains poorly studied despite its conserved presence across vertebrates. This gene is located at 12q13.13. a region occasionally linked to cancer and metabolic disorders, though its direct biological relevance is unconfirmed. Bioinformatics analyses predict C12orf43 to encode a small cytoplasmic or mitochondrial protein (~25 kDa) containing putative phosphorylation sites and α-helical domains, suggesting potential roles in signaling or structural interactions. Limited experimental data show low baseline expression in most tissues, with moderate elevation in testes and thyroid.
A 2021 proteomic study identified weak interactions with mitochondrial electron transport chain components and RNA-binding proteins, hinting at possible involvement in energy metabolism or post-transcriptional regulation. Recombinant C12orf43 has been expressed in E. coli and mammalian systems for antibody production, yet functional assays remain unreported. Challenges in characterization stem from lack of clear homologs in model organisms and absence of disease-associated variants in genomic databases.
Current research focuses on determining its subcellular localization through tagged constructs and screening for binding partners via yeast two-hybrid systems. Its inclusion in cancer biomarker discovery panels and mitochondrial disorder studies underscores growing, though speculative, interest in this orphan protein.
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