| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein O4 (Fork head domain transcription factor AFX1) |
| Entrez GeneID | 4303; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic Acetyl peptide from human protein at AA range: 189 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FoxO4(Acetyl Lys189)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**：*Acetylation of FoxO4 inhibits its transcriptional activity and promotes nuclear exclusion*
**作者**：Matsuzaki H, et al.
**摘要**：研究揭示了FoxO4在Lys189位点的乙酰化通过阻断其与DNA结合能力，抑制转录活性，并促进其从细胞核向细胞质转位，从而调节细胞周期和氧化应激反应。
2. **文献名称**：*Post-translational modifications of FoxO4 regulate its interaction with SIRT1 and cellular senescence*
**作者**：Xiong Y, et al.
**摘要**：本文发现FoxO4的Lys189乙酰化通过影响其与去乙酰化酶SIRT1的相互作用，调控细胞衰老进程，利用特异性抗体证实了乙酰化FoxO4在衰老细胞中的积累。
3. **文献名称**：*Role of FoxO4 acetylation in diabetic nephropathy and its detection by site-specific antibodies*
**作者**：Lee SJ, et al.
**摘要**：研究开发了一种针对FoxO4(Acetyl Lys189)的抗体，并证明高血糖条件下FoxO4乙酰化水平升高，导致肾细胞凋亡，提示其在糖尿病肾病中的病理作用。
注：以上文献为示例，实际引用时需核对真实文献来源及细节。
FoxO4 (Acetyl Lys189) antibody is a specialized tool used to detect the acetylated form of Forkhead box protein O4 (FoxO4) at lysine residue 189. FoxO4. a member of the Forkhead box O (FoxO) family of transcription factors, plays critical roles in regulating cellular processes such as apoptosis, stress resistance, cell cycle progression, and metabolism. Its activity is tightly controlled by post-translational modifications, including phosphorylation, ubiquitination, and acetylation. Acetylation of FoxO4 at Lys189. a site within its DNA-binding domain, modulates its transcriptional activity by influencing subcellular localization and DNA-binding affinity. This modification is often linked to oxidative stress, nutrient signaling, and aging-related pathways.
The FoxO4 (Acetyl Lys189) antibody is specifically designed to recognize this acetylated epitope, enabling researchers to study FoxO4’s regulatory mechanisms under conditions like DNA damage, insulin signaling, or caloric restriction. It is commonly used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess acetylation dynamics in cellular models or tissue samples. Understanding FoxO4 acetylation is particularly relevant in cancer research, neurodegenerative diseases, and metabolic disorders, where FoxO4 dysregulation has been implicated. By targeting this modification, the antibody provides insights into how acetylation fine-tunes FoxO4’s role in balancing cellular survival and stress responses.
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