| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/ |
| Entrez GeneID | 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968; |
| WB Predicted band size | 15kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | tri methyl peptide at K79 of Histone H3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 Histone H3 (Tri-Methyl-Lys79) 抗体的3篇参考文献概览：
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1. **"DOT1L inhibits SIRT1-mediated chromatin silencing to maintain leukemic gene expression"**
- **作者**: Bernt, K.M. 等
- **摘要**: 研究利用 H3K79me3 抗体进行染色质免疫沉淀(ChIP)，揭示了 DOT1L 介导的 H3K79 三甲基化在白血病中通过拮抗 SIRT1 去乙酰化酶维持致癌基因表达的作用，并证明其作为治疗靶点的潜力。
2. **"Regulation of chromatin structure by histone H3K79 methylation"**
- **作者**: Huyen, Y. 等
- **摘要**: 通过 H3K79me3 特异性抗体的 Western blot 和免疫荧光分析，阐明该修饰在 DNA 损伤修复中的关键调控机制，证明其与 RAD9 蛋白的相互作用维持基因组稳定性。
3. **"H3K79 methylation profiles define murine blood cell traits and defects"**
- **作者**: Kim, Y. 等
- **摘要**: 研究使用 H3K79me3 抗体进行高通量测序分析，绘制造血系统中 H3K79 三甲基化的动态图谱，揭示其在造血干细胞分化中的调控作用及与骨髓异常疾病的关联。
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以上文献均涉及 H3K79me3 抗体的实验应用(如 ChIP-seq、Western blot)，并聚焦于该修饰在基因调控、疾病机制中的功能。如需具体期刊信息或发表年份，可进一步补充。
The Histone H3 (Tri-Methyl-Lys79) antibody is a key tool for studying epigenetic regulation linked to the post-translational modification of histone H3 at lysine 79 (H3K79me3). This modification occurs within the globular core of histone H3. a region less commonly targeted compared to N-terminal tail modifications. H3K79 methylation is catalyzed by the DOT1L methyltransferase, the sole enzyme known to methylate this site in mammals. Unlike many histone marks, H3K79me3 is associated with actively transcribed gene regions and plays roles in transcriptional elongation, DNA repair, and cell cycle regulation. It is also implicated in maintaining genomic stability and has been studied in cancer, as aberrant H3K79 methylation patterns correlate with leukemia and other malignancies.
The H3K79me3 antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map methylation patterns and investigate its functional roles. Specificity validation is critical, as cross-reactivity with other methylated lysine residues or isoforms (e.g., mono- or di-methylated H3K79) can occur. Researchers must optimize conditions, considering cell type and fixation methods, to ensure accurate detection. This antibody has advanced studies in epigenetics, particularly in understanding how dynamic methylation states influence gene expression, disease mechanisms, and therapeutic targeting of DOT1L in cancers. Its applications extend to developmental biology and stem cell research, where H3K79me3 serves as a marker of transcriptional activation and chromatin state transitions.
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