| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/ |
| Entrez GeneID | 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968; |
| WB Predicted band size | 15kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | di methyl peptide at K27 of Histone H3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Histone H3 (Di-Methyl-Lys27)(H3K27me2)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：**"Role of histone H3 lysine 27 methylation in Polycomb-group silencing"**
**作者**：Cao, R., Zhang, Y.
**摘要**：本研究阐明了H3K27me2作为Polycomb抑制复合物(PRC2)介导的基因沉默标志物，通过染色质免疫沉淀(ChIP)和抗体特异性验证，证明其在胚胎干细胞分化中调控靶基因的转录抑制，并揭示了H3K27me2与染色质压缩的关联性。
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2. **文献名称**：**"Polycomb complexes repress developmental regulators in murine embryonic stem cells"**
**作者**：Boyer, L. A., et al.
**摘要**：利用H3K27me2特异性抗体进行全基因组ChIP-seq分析，发现H3K27me2广泛富集于发育调控基因的启动子区域，与PRC2复合物协同维持胚胎干细胞多能性，并抑制分化相关基因的异常激活。
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3. **文献名称**：**"Histone methylation-dependent mechanisms impose ligand dependency for gene activation by nuclear receptors"**
**作者**：Garcia-Bassets, I., et al.
**摘要**：通过H3K27me2抗体检测，揭示了该修饰在核受体(如雌激素受体)靶基因调控中的作用，证明其通过限制染色质可及性，使基因激活依赖于配体信号，为表观遗传修饰与激素依赖性疾病的关联提供了证据。
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这些研究均通过H3K27me2特异性抗体(如ChIP、Western blot等技术)解析了该修饰在基因调控、干细胞分化及疾病中的功能，可作为抗体应用的重要参考文献。
The Histone H3 (di-methyl Lys27) [H3K27me2] antibody is a critical tool for studying epigenetic regulation linked to gene expression and chromatin dynamics. Histone H3 is a core component of nucleosomes, and post-translational modifications at specific residues, such as lysine 27 (K27), play pivotal roles in modulating chromatin structure and transcriptional activity. Di-methylation at H3K27 (H3K27me2) is a moderately repressive mark associated with gene silencing, distinct from the stronger repression mediated by H3K27 trimethylation (H3K27me3), which is catalyzed by Polycomb Repressive Complex 2 (PRC2). H3K27me2 is broadly distributed across euchromatic and heterochromatic regions, potentially serving as a precursor for H3K27me3 or functioning in PRC2-independent pathways.
Antibodies targeting H3K27me2 enable researchers to map its genomic localization via ChIP-seq, assess its abundance in cellular processes like differentiation or development, and investigate its dysregulation in diseases such as cancer. Specificity is crucial, as cross-reactivity with H3K27me1 or H3K27me3 can lead to misinterpretation. Validated antibodies are essential for applications including Western blotting, immunofluorescence, and immunohistochemistry. Recent studies highlight H3K27me2's role in maintaining genomic stability and regulating lineage-specific genes, underscoring its importance in both basic and translational research. Proper controls, such as using knockout cell lines or peptide competition assays, are recommended to confirm antibody reliability.
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