Rabbit Polyclonal PCAF(Acetyl-Lys428) Antibody

The PCAF (Acetyl-Lys428) antibody is a specialized tool used to detect the acetylated form of lysine 428 (K428) on the p300/CBP-associated factor (PCAF), a histone acetyltransferase (HAT) involved in epigenetic regulation. PCAF, part of the GNAT family, acetylates histones and non-histone proteins, modulating chromatin structure and gene expression. Acetylation at K428 is a post-translational modification (PTM) that may regulate PCAF’s enzymatic activity, protein interactions, or subcellular localization, though its exact functional role remains under investigation. This site-specific antibody enables researchers to study the dynamic acetylation status of PCAF in cellular processes such as transcriptional activation, DNA repair, and cell differentiation. It is commonly used in techniques like Western blotting, immunoprecipitation, or immunofluorescence to assess acetylated PCAF levels under varying conditions (e.g., stress, differentiation, or drug treatment). Validations often include testing in PCAF-deficient cells or samples treated with deacetylase inhibitors (e.g., TSA) to confirm specificity. Dysregulation of PCAF acetylation has been implicated in diseases like cancer and neurodegenerative disorders, making this antibody valuable for exploring PCAF’s role in pathological contexts. Proper controls (e.g., unacetylated peptides or mutation models) are recommended to avoid cross-reactivity with similar epitopes.