Rabbit Polyclonal NF-E4(Acetyl-Lys43) Antibody

The NF-E4 (Acetyl-Lys43) antibody is a specialized tool used to detect the acetylated form of lysine 43 (K43) in the NF-E4 protein, a nuclear transcription factor implicated in erythroid (red blood cell) development and differentiation. NF-E4. also known as p45/NF-E2. is a subunit of the NF-E2 complex, which regulates globin gene expression and other erythroid-specific genes. Acetylation at K43 is a post-translational modification linked to chromatin remodeling and transcriptional activation, potentially enhancing DNA-binding affinity or interaction with co-regulators. This antibody is particularly valuable in studies exploring epigenetic regulation of hematopoiesis, as acetylation status often correlates with cellular differentiation stages. Researchers employ it in techniques like Western blotting, chromatin immunoprecipitation (ChIP), or immunofluorescence to investigate NF-E4's role in normal erythropoiesis, hemoglobinopathies (e.g., thalassemia), or myelodysplastic syndromes. Its specificity for the acetylated epitope makes it crucial for distinguishing active (acetylated) NF-E4 from its inactive form, providing insights into redox-sensitive signaling pathways and oxygen-responsive gene regulation in erythroid cells. Proper validation using positive/negative controls (e.g., deacetylase inhibitors like TSA) is essential due to potential cross-reactivity with other acetylated proteins.