| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5-hydroxytryptamine receptor 7; 5-HT-7; 5-HT7; 5-HT-X; Serotonin receptor 7 |
| Entrez GeneID | 3363; |
| WB Predicted band size | 53kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 5-HT7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于5-HT7抗体的3篇代表性文献，简要整理供参考：
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1. **文献名称**：*"Immunohistochemical localization of the 5-HT7 receptor in rat brain"*
**作者**：Thomas DR et al.
**摘要**：通过免疫组化技术，利用特异性5-HT7抗体研究了该受体在大鼠中枢神经系统的分布，发现其高表达于海马、丘脑和皮层，提示其参与认知和情绪调节。
2. **文献名称**：*"5-HT7 receptor inhibition and inactivation induce antidepressant-like behavior and sleep pattern"*
**作者**：Hedlund PB et al.
**摘要**：结合5-HT7抗体检测与基因敲除技术，发现抑制该受体可减少抑郁样行为并改善睡眠节律，为开发抗抑郁药物提供靶点依据。
3. **文献名称**：*"Characterization of a novel monoclonal antibody for the 5-HT7 receptor: Comparative immunohistochemical analysis in mice and humans"*
**作者**：Leopoldo M et al.
**摘要**：报道了一种新型5-HT7单克隆抗体的开发与验证，通过免疫印迹和免疫荧光确认其特异性，并对比分析了小鼠与人类组织中的受体分布差异。
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*注：若需更多文献或扩展摘要细节，可进一步补充。*
The 5-HT7 receptor, a member of the serotonin (5-hydroxytryptamine, 5-HT) receptor family, is a G protein-coupled receptor (GPCR) primarily expressed in the central nervous system, with roles in regulating mood, circadian rhythms, learning, and neuroplasticity. Antibodies targeting the 5-HT7 receptor are critical tools for studying its distribution, expression levels, and functional interactions in both physiological and pathological contexts. These antibodies, typically polyclonal or monoclonal, are designed to recognize specific epitopes on the receptor, enabling techniques like immunohistochemistry, Western blotting, and flow cytometry.
Research using 5-HT7 antibodies has linked the receptor to neurological and psychiatric disorders, including depression, anxiety, and schizophrenia, as well as potential roles in neurodevelopmental conditions. Validating antibody specificity is essential, as cross-reactivity with structurally similar receptors (e.g., 5-HT6 or 5-HT1A) can compromise data accuracy. Recent studies also explore its involvement in gut-brain axis signaling and immune modulation.
The development of selective 5-HT7 antibodies supports drug discovery efforts, particularly for therapeutics targeting serotonin-related pathways. However, challenges persist in ensuring consistent performance across experimental models (e.g., human vs. rodent tissues) and addressing isoform diversity due to alternative splicing. Ongoing advancements in antibody engineering aim to improve affinity and reduce off-target effects, enhancing their utility in both basic research and clinical applications.
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