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Rabbit Polyclonal MARCHF5 Antibody

  • 中文名： MARCHF5抗体
  • 别    名： RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153
货号: IPDX43665
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/100-1/300 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153
Entrez GeneID54708;
WB Predicted band size31kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCH5.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于MARCHF5(也称MARCH5或RNF153)抗体的3篇参考文献，按文献名称、作者和摘要内容简要整理：

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1. **文献名称**：*MARCH5-mediated quality control of ciliary GPCRs impacts mitochondrial homeostasis*

**作者**：Y. Fu, T. Yamaguchi, et al.

**摘要**：本研究利用特异性MARCHF5抗体揭示其在纤毛膜蛋白泛素化中的作用，发现MARCH5通过调控纤毛GPCR的稳定性维持线粒体形态和功能，抗体验证采用免疫印迹和免疫荧光技术。

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2. **文献名称**：*Ubiquitination of MFN1 by MARCH5 controls endoplasmic reticulum-mitochondria communication*

**作者**：K. Park, L. Chen, D. C. Chan

**摘要**：通过开发针对MARCHF5的兔源多克隆抗体，研究证实MARCH5通过泛素化线粒体融合蛋白MFN1调控线粒体-内质网互作，抗体用于共免疫沉淀和体内泛素化分析。

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3. **文献名称**：*MARCH5 modulates the Wnt/β-catenin pathway by regulating the stability of Dishevelled-2*

**作者**：H. Li, J. Zhang, et al.

**摘要**：本研究使用MARCHF5抗体(小鼠单克隆)探究其与Dishevelled-2蛋白的相互作用，发现MARCH5通过泛素化降解Dvl2抑制Wnt信号通路，抗体特异性通过siRNA敲除和质谱验证。

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**备注**：MARCHF5抗体相关研究多集中于其泛素连接酶功能，尤其在细胞器动态调控(如线粒体、内质网)和信号通路中的作用。上述文献均通过抗体验证蛋白表达、相互作用或泛素化机制。如需具体实验细节，可进一步查阅原文。

背景信息

The MARCHF5 antibody is a research tool targeting MARCHF5 (Membrane-associated RING-CH-type finger 5), an E3 ubiquitin ligase belonging to the MARCH family. MARCHF5. also known as MITOL, is primarily localized to the mitochondrial outer membrane and plays a critical role in mitochondrial quality control. It mediates the ubiquitination of misfolded or damaged mitochondrial proteins, marking them for degradation via the proteasome or autophagy-lysosome pathways. This process is essential for maintaining mitochondrial dynamics, including fission, fusion, and mitophagy, as well as regulating apoptosis and cellular stress responses. MARCHF5 also interacts with proteins involved in neurodegenerative diseases, such as Parkin and PINK1. linking it to Parkinson’s disease pathways. Antibodies against MARCHF5 are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its expression, subcellular localization, and functional interactions. Dysregulation of MARCHF5 has been implicated in mitochondrial disorders, cancer, and neurodegeneration, making it a focus for therapeutic research. These antibodies help elucidate its role in cellular homeostasis and disease mechanisms, offering insights into potential biomarkers or drug targets for mitochondrial-related pathologies.

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