Rabbit Polyclonal KLHL3 Antibody

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     Western blot analysis of GPR180 expression in Jurkat, HEK293T whole cell lysates.    

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     Immunofluorescent analysis of KLHL3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.    

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     Immunohistochemical analysis of KLHL3 staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.    

KLHL3 (Kelch-like family member 3) is a substrate-recognition component of the Cullin 3 (CUL3)-based E3 ubiquitin ligase complex, which plays a critical role in protein ubiquitination and degradation. It is primarily known for regulating electrolyte homeostasis by targeting WNK (With No Lysine) kinases for ubiquitin-dependent proteolysis. Mutations in KLHL3 are linked to familial hypertension disorders, such as pseudohypoaldosteronism type II (PHAII), also known as Gordon syndrome, characterized by hyperkalemia, hypertension, and metabolic acidosis. Research on KLHL3 has expanded its implications to renal and cardiovascular pathophysiology, particularly in sodium and potassium balance.

KLHL3 antibodies are essential tools for studying its expression, localization, and interaction partners in cellular and tissue contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to explore KLHL3's regulatory mechanisms in disease models. Commercial KLHL3 antibodies are typically raised against specific epitopes (e.g., N-terminal BTB domain or C-terminal Kelch repeats) and validated for species reactivity (human, mouse, rat). Challenges include ensuring specificity due to structural similarities within the Kelch-like protein family. Recent studies also investigate KLHL3's role beyond ion transport, including tumor suppression and immune modulation, broadening its therapeutic relevance. Reliable KLHL3 antibodies thus underpin both basic research and translational studies targeting hypertension and related disorders.