| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/ 50-300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 400823; |
| WB Predicted band size | 18kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FAM177B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于公开数据库生成的模拟参考文献示例，实际文献可能需要通过学术数据库进一步检索验证：
1. **文献名称**: "Characterization of FAM177B as a novel mitochondrial protein involved in cellular stress response"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫沉淀和Western blot技术，利用FAM177B抗体验证了该蛋白在线粒体中的定位，并发现其在氧化应激条件下的表达上调，提示其可能参与细胞应激调控通路。
2. **文献名称**: "FAM177B interacts with PI3K-AKT signaling pathway and promotes cancer cell survival"
**作者**: Lee S, et al.
**摘要**: 通过免疫组织化学(IHC)分析，发现FAM177B在多种肿瘤组织中高表达，且其抗体特异性验证显示与PI3K复合物存在共定位，表明其可能通过AKT信号通路抑制细胞凋亡。
3. **文献名称**: "The role of FAM177B in neurodevelopmental disorders: Insights from knockout mouse models"
**作者**: Thompson R, et al.
**摘要**: 利用FAM177B特异性抗体进行脑组织免疫荧光染色，发现该蛋白在小鼠神经元前体细胞中高表达，基因敲除后导致皮质发育异常，提示其在神经发育中的潜在作用。
4. **文献名称**: "Proteomic screening identifies FAM177B as a binding partner of c-Myc"
**作者**: Gupta A, et al.
**摘要**: 通过Co-IP联合质谱分析，发现FAM177B与致癌蛋白c-Myc存在相互作用，抗体阻断实验进一步证实二者在细胞周期调控中的协同功能。
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**备注**：以上文献为模拟生成，实际研究中FAM177B相关抗体文献较为有限。建议通过PubMed、Google Scholar等平台以“FAM177B antibody”或“FAM177B function”为关键词检索最新进展。若需具体文献，可提供更详细的研究方向以便进一步筛选。
The FAM177B (Family With Sequence Similarity 177 Member B) antibody is a tool used to study the poorly characterized protein encoded by the FAM177B gene. This gene is conserved across species and has been linked to cellular processes such as cell cycle regulation, DNA repair, or intracellular signaling, though its precise biological function remains unclear. FAM177B is ubiquitously expressed, with higher levels observed in tissues like the brain, liver, and kidney. Dysregulation of FAM177B has been tentatively associated with cancers, neurodevelopmental disorders, and immune-related conditions, though mechanistic insights are limited.
The FAM177B antibody, typically developed in hosts like rabbits or mice, can be monoclonal or polyclonal and is designed to target specific epitopes of the protein. It is utilized in techniques such as Western blotting (WB), immunohistochemistry (IHC), or immunofluorescence (IF) to detect FAM177B expression and localization in cells or tissues. Commercial antibodies often undergo validation using knockout controls or siRNA silencing to confirm specificity, though cross-reactivity risks may exist due to incomplete functional annotation of the protein.
Current research with FAM177B antibodies focuses on elucidating its role in disease pathways and cellular homeostasis. However, the antibody's utility is constrained by the limited understanding of FAM177B's interactions and physiological context. Further studies are needed to standardize its application and explore its potential as a diagnostic or therapeutic target.
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