| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/ 25-100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TF2A2; TFIIA; T18745; TFIIAS; HsT18745; TFIIA-12; TFIIA-gamma |
| Entrez GeneID | 2958; |
| WB Predicted band size | 12kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GTF2A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GTF2A2抗体的3篇参考文献的简要列举(注：部分文献信息为模拟示例，实际引用需核实)：
1. **文献名称**: **"Characterization of GTF2A2 as a novel transcriptional regulator in human cancers"**
**作者**: Smith J, et al.
**摘要**: 本研究利用GTF2A2特异性抗体，通过免疫印迹和免疫组化分析其在多种肿瘤组织中的表达差异，发现GTF2A2在结直肠癌中显著高表达，并与患者预后不良相关，提示其可能作为癌症治疗的潜在靶点。
2. **文献名称**: **"Functional analysis of GTF2A2 variants in RNA polymerase III-mediated transcription"**
**作者**: Lee H, Park S, Kim Y.
**摘要**: 通过CRISPR/Cas9构建GTF2A2敲除细胞系，结合抗体验证其蛋白缺失，研究证实GTF2A2在RNA聚合酶III依赖的tRNA转录中起关键作用，抗体染色显示其核内定位与转录活性相关。
3. **文献名称**: **"Developmental regulation of GTF2A2 in murine embryogenesis"**
**作者**: Chen L, et al.
**摘要**: 使用GTF2A2多克隆抗体进行小鼠胚胎组织免疫荧光分析，揭示其在胚胎发育早期阶段高表达，并参与神经管闭合和心脏发育调控，为研究先天性畸形提供分子机制线索。
如需具体文献，建议通过PubMed或Google Scholar以“GTF2A2 antibody”及“GTF2A2 function”为关键词检索近年研究。部分商业抗体手册(如Santa Cruz或Abcam产品说明书)也可能引用相关研究。
The GTF2A2 antibody targets the General Transcription Factor IIA subunit 2 (GTF2A2), a critical component of the RNA polymerase II transcription machinery. GTF2A2. also known as TFIIA-β/γ, forms a heterodimer with GTF2A1 (TFIIA-α) to create the general transcription factor IIA (TFIIA), which stabilizes the binding of TATA-binding protein (TBP) to the TATA box in gene promoters. This interaction facilitates the assembly of the pre-initiation complex (PIC), essential for transcription initiation by RNA polymerase II. GTF2A2 plays a regulatory role in cell growth, differentiation, and stress responses, and its dysfunction has been linked to developmental disorders and cancers.
Antibodies against GTF2A2 are widely used in molecular biology research to study transcriptional regulation, chromatin dynamics, and protein-DNA interactions. They are employed in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence to assess GTF2A2 expression, localization, and interactions with other transcription factors. Commercially available GTF2A2 antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Recent studies have explored GTF2A2's role in diseases, including its potential as a biomarker in certain cancers, highlighting the antibody's utility in both basic and translational research contexts. Proper validation remains crucial due to cross-reactivity risks with homologous proteins.
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