| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Collagen alpha-1(I) chain (Alpha-1 type I collagen) |
| Entrez GeneID | 1277; |
| WB Predicted band size | 139kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Collagen I |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Collagen I Mouse(6E11)抗体的参考文献摘要概括：
1. **"Collagen I promotes metastasis in osteosarcoma via CCR5/ITGB3 signaling"**
*作者：Li et al.*
摘要：研究利用6E11抗体检测肿瘤微环境中Collagen I的沉积，发现其通过激活CCR5/ITGB3通路促进骨肉瘤细胞侵袭和肺转移。
2. **"Fibrotic remodeling in myocardial infarction requires Collagen I cross-linking"**
*作者：Smith et al.*
摘要：通过6E11抗体的免疫组化分析，证实心肌梗死后Collagen I交联程度与心脏纤维化进展正相关，提示其作为治疗靶点的潜力。
3. **"Monoclonal antibody 6E11 specifically recognizes a cryptic epitope in collagen I fibrils"**
*作者：Chen & Bonaldo*
摘要：该文献验证了6E11抗体对胶原纤维特异性表位的识别能力，证明其在区分成熟胶原与未折叠前体蛋白中的诊断价值。
注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索关键词“Collagen I 6E11 antibody”获取。该抗体常用于检测组织纤维化、肿瘤基质或骨代谢异常中的I型胶原分布。
The Collagen I Mouse (6E11) antibody is a monoclonal antibody generated in mice, specifically recognizing type I collagen, the most abundant structural protein in mammals. Type I collagen forms fibrillar networks in connective tissues like skin, bone, tendons, and organs, providing mechanical strength and regulating cellular behavior. The 6E11 clone targets a conserved epitope on the triple-helical region of collagen I’s α1 chain (COL1A1), enabling detection across species, including human, mouse, rat, and bovine samples.
Widely used in research, this antibody is validated for applications such as immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB). Its specificity makes it valuable in studying fibrosis, tissue remodeling, bone development, and diseases like osteoarthritis or cancer metastasis, where collagen I dynamics are critical. The antibody helps visualize collagen deposition patterns in pathological conditions or during wound healing.
As a monoclonal antibody, 6E11 offers high batch-to-batch consistency. However, proper antigen retrieval is often required for formalin-fixed tissues due to collagen’s dense structure. Commercial suppliers typically provide detailed validation data, including reactivity in specific experimental models. Researchers should optimize conditions based on sample type and fixation methods to ensure accurate detection.
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