Mouse Monoclonal EpsilonTubulinMouse(3G1) Antibody

The Epsilon Tubulin Mouse (3G1) monoclonal antibody is a well-characterized tool designed to specifically detect the ε-isoform of tubulin, a less commonly studied member of the tubulin superfamily. Tubulins are essential cytoskeletal proteins involved in critical cellular processes like mitosis, intracellular transport, and cell shape maintenance. While α- and β-tubulins form heterodimers in microtubules, ε-tubulin localizes to the centrosome and plays a specialized role in centriole assembly, basal body formation, and ciliogenesis. The 3G1 clone, generated by immunizing mice with purified ε-tubulin protein or peptide fragments, exhibits high specificity for ε-tubulin across human, mouse, and rat samples, with minimal cross-reactivity to other tubulin isoforms. Validated in applications such as Western blotting, immunofluorescence, and immunoprecipitation, this antibody has been instrumental in elucidating ε-tubulin's function in cell cycle regulation, particularly during centriole duplication and mitotic spindle orientation. Its use has advanced studies in developmental biology, cilia-related disorders, and cancer research, where centrosomal abnormalities are linked to genomic instability. The antibody’s reliability in distinguishing ε-tubulin from γ-tubulin (another centrosomal tubulin) makes it a key reagent for probing microtubule-organizing center dynamics.