| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 7444; |
| WB Predicted band size | 58kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human VRK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VRK2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Development and Validation of a VRK2 Monoclonal Antibody for Immunoblotting and Immunohistochemical Analysis"*
**作者**: Kim, J.H., et al.
**摘要**: 该研究报道了一种新型小鼠源单克隆抗体的开发，该抗体针对人类VRK2蛋白的C末端结构域。通过Western blot和免疫组化验证了其在多种癌细胞系及正常组织中的特异性，证实其在检测VRK2表达水平及定位中的应用价值。
2. **文献名称**: *"VRK2 Antibody-Based Profiling Reveals Differential Expression in Glioblastoma Multiforme"*
**作者**: Rodriguez-Pinilla, M., et al.
**摘要**: 作者利用商业化VRK2抗体(货号ab12345)，通过免疫组织化学分析脑胶质瘤样本，发现VRK2在胶质母细胞瘤中显著高表达，并与患者生存率负相关，提示其作为潜在生物标志物的可能性。
3. **文献名称**: *"Technical Evaluation of Commercial VRK2 Antibodies for Reliable Detection in Mitochondrial Fractions"*
**作者**: Wang, Q., et al.
**摘要**: 研究系统性评估了5种市售VRK2抗体的性能，发现部分抗体存在交叉反应性(如与VRK1交叉)。推荐使用克隆号2A3的抗体，因其在亚细胞线粒体组分检测中表现出高特异性和灵敏度。
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**备注**：以上文献为示例，实际引用时请核对真实来源。建议通过PubMed或Google Scholar以关键词“VRK2 antibody validation”或“VRK2 immunohistochemistry”检索最新研究。
The VRK2 (Vaccinia-Related Kinase 2) antibody is a tool used to study the VRK2 protein, a serine/threonine kinase belonging to the VRK family, which plays roles in cell cycle regulation, DNA damage response, and nuclear envelope assembly. VRK2 exists in two isoforms: a longer cytoplasmic form (VRK2A) and a shorter nuclear variant (VRK2B). Dysregulation of VRK2 has been implicated in neurological disorders (e.g., schizophrenia, depression) and cancers (e.g., glioblastoma, breast cancer), where it may promote tumor progression by suppressing apoptosis or modulating signaling pathways like Wnt/β-catenin.
Antibodies targeting VRK2 are essential for detecting its expression, localization, and interactions in research models. They are commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Commercial VRK2 antibodies are typically validated using knockout cell lines or siRNA-mediated silencing to ensure specificity. Recent studies highlight VRK2's involvement in stress responses, neurodevelopment, and chemoresistance, making it a potential biomarker or therapeutic target. However, challenges remain in understanding its isoform-specific functions and regulatory mechanisms, driving ongoing demand for reliable VRK2 antibodies in biomedical research.
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