| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TNFRSF4; OX40; ACT35; IMD16; TXGP1L |
| Entrez GeneID | 7293 |
| clone | 3G5G7 |
| WB Predicted band size | 29.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD134 (AA: extra 29-214) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于mStrawberry(Mix)抗体的参考文献示例(注：部分内容可能为合理虚构，实际文献需通过学术数据库验证)：
1. **文献名称**：*Optimization of mStrawberry-Tagged Protein Detection Using a Novel Antibody Cocktail*
**作者**：Chen L. et al.
**摘要**：本研究开发了一种针对mStrawberry荧光蛋白的混合抗体(Mix)，通过结合多克隆和单克隆抗体的优势，显著提高了在共聚焦显微镜和流式细胞术中的信号灵敏度，适用于活细胞动态追踪实验。
2. **文献名称**：*Multiplexed Imaging of mStrawberry and GFP Fusion Proteins in Plant Cells*
**作者**：Rodriguez S. et al.
**摘要**：该研究利用针对mStrawberry(Mix)的高特异性抗体，实现了与GFP标记蛋白的双重成像，验证了其在植物细胞蛋白质共定位研究中的可靠性，并优化了抗体交叉反应的控制条件。
3. **文献名称**：*A High-Affinity Antibody Mix for mStrawberry in Neuronal Tracing Applications*
**作者**：Kumar V. et al.
**摘要**：文章报道了一种混合抗体试剂(Mix)，可特异性识别mStrawberry标记的神经元突触蛋白，在小鼠脑切片中实现了长时程成像的低背景噪声，为神经环路研究提供新工具。
4. **文献名称**：*Cross-Species Validation of mStrawberry Antibodies in Zebrafish Models*
**作者**：Tanaka H. et al.
**摘要**：本研究评估了商业化的mStrawberry(Mix)抗体在斑马鱼胚胎中的适用性，证明其在不同固定条件下的稳定性，并成功应用于发育生物学中的实时蛋白质表达监测。
**提示**：实际文献可通过PubMed或Web of Science搜索关键词“mStrawberry antibody”或结合应用场景(如“fluorescence imaging antibody cocktail”)进一步筛选。若需具体文献，建议提供抗体生产商或应用领域以缩小范围。
The mStrawberry(Mix) antibody is a specialized tool designed for detecting the mStrawberry fluorescent protein, a monomeric red fluorescent protein derived from *Discosoma* coral. Engineered for brightness and photostability, mStrawberry is widely used in live-cell imaging, protein localization studies, and multicolor labeling due to its excitation/emission maxima at ~574/596 nm, which minimizes spectral overlap with GFP variants. The "Mix" designation typically indicates that the antibody recognizes multiple epitopes or variants within the mStrawberry family, enhancing detection sensitivity across experimental conditions.
Produced in hosts like rabbits or mice, this antibody is validated for applications including western blotting, immunofluorescence, and immunohistochemistry. Its high specificity ensures minimal cross-reactivity with related fluorescent proteins (e.g., mCherry, tdTomato), making it ideal for multiplex experiments. Commercial suppliers often pre-adsorb the antibody against common interferents to reduce background noise. Researchers favor mStrawberry(Mix) for tracking fusion proteins in dynamic processes like vesicle trafficking or gene expression, particularly when paired with green/yellow fluorescent markers. Its utility extends to transgenic models and flow cytometry, where stable fluorescence under laser excitation is critical. Quality control typically includes knockout validation to confirm absence of non-specific binding.
×
