| WB | 咨询技术 | Dm |
| IF | 咨询技术 | Dm |
| IHC | 咨询技术 | Dm |
| ICC | 技术咨询 | Dm |
| FCM | 咨询技术 | Dm |
| Elisa | 咨询技术 | Dm |
| Aliases | Luciferase |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Dm |
| Immunogen | Synthetic Peptide |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于Luciferase Mouse(9D6)抗体的3篇参考文献示例(注：文献为示例性概括，实际引用需根据真实文献调整)：
---
1. **"Application of Luciferase (9D6) Monoclonal Antibody in Reporter Gene Assay Validation"**
*Authors: Smith A, et al.*
摘要：研究利用Luciferase Mouse(9D6)抗体通过Western blot和免疫荧光技术，验证了荧光素酶报告基因系统在细胞转染实验中的稳定性和灵敏度，证明该抗体适用于定量检测荧光素酶表达水平。
---
2. **"In Vivo Imaging of Luciferase-Tagged Tumors Using 9D6 Antibody-Based Detection"**
*Authors: Chen L, et al.*
摘要：通过Luciferase Mouse(9D6)抗体的免疫组化分析，实现了对小鼠肿瘤模型中荧光素酶标记细胞的定位和追踪，为活体成像技术提供了高特异性的分子标记验证方法。
---
3. **"Optimizing Luciferase Detection in Viral Vector Systems with 9D6 Clone"**
*Authors: García-Rodríguez F, et al.*
摘要：研究评估了Luciferase Mouse(9D6)抗体在腺病毒载体表达检测中的应用，优化了ELISA和流式细胞术的检测条件，显著提高了低浓度荧光素酶的检出限。
---
**备注**：实际文献需通过PubMed或Google Scholar等平台，以关键词“Luciferase 9D6 antibody”或“anti-Luciferase clone 9D6”检索具体研究。部分相关抗体厂商(如Santa Cruz Biotechnology)的技术手册也可能提供应用案例。
The Luciferase Mouse(9D6) antibody is a monoclonal antibody generated against firefly (Photinus pyralis) luciferase, a widely used bioluminescent reporter enzyme in molecular and cellular biology. Clone 9D6. derived from mouse hybridoma cells, specifically recognizes the 61 kDa luciferase protein and exhibits high affinity and specificity, with minimal cross-reactivity to endogenous mammalian proteins. It has been validated for applications including Western blotting, immunoprecipitation, immunofluorescence, and ELISA, enabling precise detection and quantification of luciferase-tagged proteins in engineered cell lines or transgenic models.
This antibody is particularly valuable in studies tracking gene expression dynamics, protein localization, or promoter activity via luciferase-based reporter assays. Its utility extends to drug discovery, where luciferase systems monitor compound effects on cellular pathways. The 9D6 clone is often preferred for its consistent performance in detecting low-abundance luciferase fusion proteins, even in complex biological samples. Commercial versions are typically supplied as purified IgG fractions, optimized for compatibility with common laboratory protocols. As luciferase assays are non-destructive, pairing this antibody with luciferin substrates allows longitudinal studies, making it a staple in fields like oncology, immunology, and gene therapy research.
×
