| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAP; PDCD9; S30mt; MRP-S30 |
| Entrez GeneID | 10884; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MRPS30 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPS3抗体的示例参考文献(内容为虚构，仅作格式参考)：
1. **文献名称**：*MRPS3 modulates mitochondrial translation and apoptosis in cancer cells*
**作者**：Chen L, et al.
**摘要**：本研究通过MRPS3抗体检测发现，MRPS3在多种癌细胞中高表达，敲低MRPS3会抑制线粒体蛋白质合成并诱导细胞凋亡，提示其作为癌症治疗靶点的潜力。
2. **文献名称**：*Antibody-based profiling of MRPS3 in neurodegenerative disorders*
**作者**：Smith JR, et al.
**摘要**：利用特异性MRPS3抗体分析阿尔茨海默病患者脑组织，发现MRPS3在线粒体中的表达异常，可能与神经元能量代谢障碍相关。
3. **文献名称**：*MRPS3 interacts with viral proteins and regulates innate immunity*
**作者**：Wang Y, et al.
**摘要**：通过免疫共沉淀(Co-IP)和Western blot(使用MRPS3抗体)，揭示了MRPS3与RNA病毒蛋白的相互作用，影响宿主抗病毒信号通路。
4. **文献名称**：*Development and validation of a high-affinity monoclonal MRPS3 antibody*
**作者**：Kim H, et al.
**摘要**：报道了一种新型MRPS3单克隆抗体的制备与验证，该抗体在免疫组化和流式细胞术中表现高特异性，适用于线粒体功能研究。
(注：以上文献为示例，实际引用需以真实发表研究为准。)
**Background of MRPS3 Antibody**
Mitochondrial Ribosomal Protein S3 (MRPS3) is a key component of the mitochondrial small ribosomal subunit (28S), essential for mitochondrial protein synthesis. Encoded by nuclear DNA, MRPS3 is synthesized in the cytoplasm and transported to mitochondria, where it contributes to the assembly and stability of the mitochondrial ribosome. This ribosome specializes in translating mitochondrial DNA (mtDNA)-encoded mRNAs, particularly those critical for oxidative phosphorylation (OXPHOS) complexes, which drive cellular energy production.
MRPS3 antibodies are immunological tools designed to detect and quantify MRPS3 expression in research applications. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study mitochondrial dysfunction, ribosome biogenesis, and cellular energy metabolism. Dysregulation of MRPS3 has been implicated in mitochondrial diseases, cancer, and neurodegenerative disorders, as impaired mitochondrial translation disrupts ATP production and promotes oxidative stress.
Studies also link MRPS3 to apoptosis regulation, as its depletion can trigger mitochondrial-mediated cell death. Additionally, MRPS3 mutations or altered expression are observed in conditions like Leigh syndrome and certain carcinomas, highlighting its diagnostic and therapeutic research potential. Commercial MRPS3 antibodies are typically validated for specificity using knockout controls or siRNA-based silencing to ensure accurate detection. These antibodies enable insights into mitochondrial biology, disease mechanisms, and metabolic adaptations in diverse physiological contexts.
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