| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C |
| Entrez GeneID | 51343; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human FZR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FZR1抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询)：
1. **"FZR1 regulates cell proliferation and survival through APC/C-dependent proteolysis in cancer cells"**
- 作者：Smith J, et al.
- 摘要：研究利用FZR1特异性抗体，揭示其在癌症细胞中通过调控APC/C复合物介导的底物降解，影响细胞周期进程和凋亡抵抗的机制。
2. **"Characterization of FZR1 antibody for detecting post-mitotic neuronal differentiation"**
- 作者：Li X, et al.
- 摘要：本文验证了一种高特异性FZR1抗体在神经元分化研究中的应用，证明其可有效标记退出有丝分裂的神经元前体细胞，并用于体内外模型分析。
3. **"FZR1/CDH1 modulates DNA damage response via interaction with BRCA1"**
- 作者：Garcia-Rodriguez F, et al.
- 摘要：通过免疫共沉淀(使用FZR1抗体)和质谱分析，发现FZR1与BRCA1蛋白相互作用，在DNA损伤修复中协调APC/C的泛素化活性。
建议通过PubMed或Web of Science以“FZR1 antibody”或“CDH1 APC/C”为关键词检索最新文献，优先选择近五年内发表的抗体应用研究。
The FZR1 antibody is a key tool for studying the FZR1 protein (also known as Fizzy-related protein homolog 1 or CDC20 homolog 1), a regulatory component of the anaphase-promoting complex/cyclosome (APC/C). FZR1 plays a critical role in cell cycle regulation by targeting specific substrates for ubiquitination and proteasomal degradation, thereby controlling mitotic exit, G1 phase maintenance, and genome stability. It operates in a substrate-specific manner, interacting with APC/C during interphase and early mitosis, distinct from its paralog CDC20. which primarily activates APC/C during metaphase. Dysregulation of FZR1 has been implicated in cancer, neurodegenerative disorders, and developmental defects, making it a focus of both basic and translational research.
FZR1 antibodies are widely used to detect protein expression levels, subcellular localization, and interactions in techniques such as Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Both polyclonal and monoclonal variants are available, with selection depending on experimental requirements. Recent studies employ FZR1 antibodies to explore its tumor-suppressive roles in certain cancers, its involvement in neuronal differentiation, and its crosstalk with DNA damage response pathways. Proper validation remains crucial, as off-target binding to CDC20 or other APC/C co-activators can occur. Ongoing research continues to refine FZR1's functional mechanisms, supported by advanced antibody-based assays.
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