Rabbit Polyclonal PDZK1 Antibody

PDZK1 (Post-synaptic density protein 95/Discs large/Zonula occludens-1 containing protein 1) is a multi-PDZ domain scaffolding protein that plays a critical role in regulating membrane transport proteins, particularly in epithelial cells of the kidneys, intestines, and liver. It interacts with various ion channels, transporters (e.g., Na+/H+ exchanger regulatory factor 3. NHE3), and receptors to modulate their localization, stability, and activity. PDZK1 is essential for maintaining electrolyte balance, nutrient absorption, and drug metabolism pathways. Research on PDZK1 antibodies has grown due to their utility in studying protein-protein interactions and disease mechanisms. These antibodies, often developed in rabbits or mice using immunogenic peptides from conserved PDZK1 regions, enable detection of PDZK1 in Western blotting, immunohistochemistry, and co-immunoprecipitation assays. Studies using PDZK1 antibodies have linked the protein to hypertension, hypercholesterolemia, and cancer progression, where its dysregulation affects signaling pathways like MAPK/ERK. Knockout mouse models validated its physiological relevance, showing metabolic defects and altered drug sensitivity. Commercial PDZK1 antibodies are typically validated for specificity using knockdown cells or tissue samples. Ongoing research focuses on PDZK1’s role as a potential therapeutic target or biomarker, particularly in disorders of membrane transport and cellular signaling.