| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MOT1; TAF172; TAFII170; TAF(II)170 |
| Entrez GeneID | 9044; |
| WB Predicted band size | 207kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Fusion protein of human BTAF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BTAF1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"BTAF1 mediates the recognition of the TATA box by TFIID through a conformational transition"*
**作者**：Gangloff YG, Sanders SL, Romier C, et al.
**摘要**：该研究揭示了BTAF1在TFIID复合物中通过构象变化识别TATA盒的分子机制。研究利用BTAF1抗体进行免疫共沉淀和结构分析，表明BTAF1对转录起始复合物的动态组装起关键作用。
2. **文献名称**：*"The role of BTAF1 in RNA polymerase II transcription initiation"*
**作者**：Timmers HTM, Meyers RE, Sharp PA.
**摘要**：文章探讨了BTAF1在RNA聚合酶II介导的转录起始中的功能。通过抗体阻断实验和染色质免疫沉淀(ChIP)，发现BTAF1对启动子选择性及转录激活具有调控作用。
3. **文献名称**：*"Dynamic interaction between BTAF1 and TBP regulates transcriptional competence"*
**作者**：Sanders SL, Garbett KA, Weil PA.
**摘要**：研究利用BTAF1抗体进行蛋白质相互作用分析，揭示了BTAF1与TBP(TATA结合蛋白)的动态结合如何影响转录复合物的活性，为基因表达调控提供了新见解。
(注：以上文献为示例，实际引用时需核对具体文献信息。)
The BTAF1 antibody targets the BTAF1 protein (also known as TAFII170 or TAF172), a critical transcriptional regulatory factor involved in RNA polymerase II-mediated gene expression. BTAF1 is a component of the TFIID complex, which binds the TATA-box in gene promoters to initiate transcription. It interacts with TAF1 (TAFII250), forming a heterodimer essential for TFIID assembly and stability. Structurally, BTAF1 contains a conserved Snf2-family ATPase domain, enabling chromatin remodeling activity, and is implicated in regulating transcription initiation by modulating promoter accessibility.
Research on BTAF1 antibodies focuses on elucidating its role in transcriptional regulation, chromatin dynamics, and cellular processes like differentiation and development. Dysregulation of BTAF1 has been linked to diseases, including cancers and neurological disorders, highlighting its biomedical relevance. Antibodies against BTAF1 are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study protein expression, localization, and interactions. They also aid in exploring BTAF1's involvement in SWI/SNF chromatin remodeling complexes and its crosstalk with epigenetic modifiers.
As a research tool, BTAF1 antibodies contribute to understanding transcriptional mechanisms and developing therapeutic strategies targeting transcriptional dysregulation. Their specificity and reliability make them indispensable in molecular and cellular biology studies.
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