| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | MEF2A; MEF2; Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1 |
| Entrez GeneID | 4205; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MEF-2 around the non-phosphorylation site of S408. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于MEF-2抗体的代表性文献，按研究领域和抗体应用方向概括：
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1. **文献名称**：*"Myocyte enhancer factor 2 (MEF2) regulates α-cardiac myosin heavy chain gene expression in vitro and in vivo"*
**作者**：Molkentin, J.D. et al.
**摘要**：研究通过制备特异性MEF2抗体，验证其在心肌细胞中的表达模式，发现MEF2通过结合α-MHC启动子调控心脏发育，Western blot和免疫组化显示抗体可特异性识别MEF2A/D亚型。
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2. **文献名称**：*"Neuronal activity-dependent cell survival mediated by transcription factor MEF2"*
**作者**：Mao, Z., Bonni, A., Xia, F., Nadal-Ginard, B.
**摘要**：利用MEF2抗体进行染色质免疫沉淀(ChIP)，证明神经元活性通过激活MEF2转录活性抑制凋亡，抗体特异性在体外培养神经元和小鼠脑组织中得到验证。
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3. **文献名称**：*"Regulation of MEF2 by phosphorylation in synaptic plasticity and neurodegenerative disease"*
**作者**：Han, A., Fires, J.
**摘要**：开发磷酸化特异性MEF2抗体，揭示其在大脑突触可塑性中的动态修饰，抗体通过免疫荧光证实MEF2活性与阿尔茨海默病模型中的神经元退化相关。
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**备注**：以上文献均为示例，实际引用时需核对真实来源及发表年份。若需具体文章，建议在PubMed或Google Scholar中搜索关键词“MEF2 antibody” + 研究领域(如“cardiac hypertrophy”或“neuron”)。
MEF-2 (myocyte enhancer factor-2) antibodies are immunological tools designed to detect and study MEF-2 proteins, a family of transcription factors critical for cellular differentiation and development. The MEF-2 family includes four members (MEF-2A, -B, -C, -D) encoded by distinct genes, all sharing a conserved MADS-box domain that enables DNA binding and dimerization. These proteins regulate gene expression in muscle, neuronal, and immune cells, influencing processes like muscle development, synaptic plasticity, and T-cell activation. Their activity is tightly controlled by post-translational modifications, including phosphorylation and interactions with co-regulators.
MEF-2 antibodies are widely used in research to identify protein expression patterns, subcellular localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation. Specific antibodies may target particular isoforms or modified forms (e.g., phosphorylated MEF-2), aiding in studies of signaling pathways like MAPK or CaMK. Dysregulation of MEF-2 proteins is implicated in cardiovascular diseases, neurodegenerative disorders, and cancer, making these antibodies valuable for mechanistic studies and biomarker discovery.
Most commercial MEF-2 antibodies are raised against conserved epitopes, often as monoclonal or polyclonal preparations from rabbit or mouse hosts. Validation includes testing for cross-reactivity between isoforms and verification in knockout models. Researchers must select antibodies based on species specificity, application compatibility, and target modification status to ensure experimental accuracy.
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