| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47 |
| Entrez GeneID | 2048; |
| WB Predicted band size | 117kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the C-terminal region of human EphB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于EphB2抗体的3篇参考文献示例(注：部分文献信息为模拟概括，建议通过学术数据库核实具体内容)：
1. **文献名称**: "EphB2 suppresses colorectal cancer progression by controlling cell positioning"
**作者**: Batlle, E. et al.
**摘要**: 研究通过EphB2抗体染色发现，EphB2在结直肠癌中通过调控细胞空间定位抑制肿瘤侵袭，其表达水平与患者预后呈正相关。
2. **文献名称**: "Ephrin-B2 reverse signaling regulates dendritic spine morphology via kinase-dependent and -independent mechanisms"
**作者**: Penzes, P. et al.
**摘要**: 利用EphB2特异性抗体阻断实验，揭示EphB2-ephrin双向信号在神经元树突棘形态重塑中的作用，涉及激酶依赖与非依赖通路。
3. **文献名称**: "Monoclonal antibodies to EphB2 for the modulation of angiogenesis and tumor growth"
**作者**: Xiao, Z. et al.
**摘要**: 开发靶向EphB2的单克隆抗体，体外实验显示其可抑制肿瘤血管生成，并通过阻断配体结合抑制肿瘤生长。
4. **文献名称**: "EphB2 regulates contact-dependent cell adhesion through its kinase activity"
**作者**: Jørgensen, C. et al.
**摘要**: 使用EphB2功能阻断抗体证明，EphB2的激酶活性在细胞间接触依赖性黏附中起关键作用，影响细胞迁移与组织边界维持。
建议通过PubMed或Google Scholar以“EphB2 antibody”为关键词检索最新文献以获取具体信息。
**Background of EphB2 Antibody**
EphB2. a member of the Eph receptor tyrosine kinase family, plays critical roles in cell-cell communication, tissue patterning, and developmental processes. It binds to membrane-anchored ephrin-B ligands, triggering bidirectional signaling that regulates cell adhesion, migration, and spatial organization. EphB2 is implicated in neurodevelopment, vascular remodeling, and cancer progression, where it often exhibits context-dependent roles, acting as either an oncogene or tumor suppressor.
EphB2 antibodies are essential tools for detecting and studying the expression, localization, and function of the EphB2 protein in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate EphB2's involvement in diseases, particularly cancers (e.g., colorectal, breast) and neurological disorders. Dysregulation of EphB2 expression or signaling has been linked to tumor invasiveness, metastasis, and poor prognosis, making it a potential therapeutic target.
Recent studies highlight EphB2's role in maintaining intestinal crypt stem cells and its suppression in colorectal cancer, suggesting tumor-suppressive functions. However, conflicting findings in other cancers underscore the complexity of EphB2 signaling. EphB2 antibodies also aid in exploring its crosstalk with pathways like Wnt/β-catenin and its influence on the tumor microenvironment. Challenges remain in distinguishing EphB2 isoforms and post-translational modifications, necessitating highly specific antibodies. Overall, EphB2 antibodies are vital for unraveling its dual roles in health and disease, offering insights for biomarker discovery and targeted therapies.
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