| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rab11-FIP3; EF hands-containing Rab-interacting protein; Eferin; |
| Entrez GeneID | 9727; |
| WB Predicted band size | 83kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human RAB11FIP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB11FIP3抗体的3篇参考文献，涵盖其功能研究和应用：
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1. **文献名称**：Rab11-FIP3 regulates endosomal recycling dynamics and cell polarity during tissue morphogenesis
**作者**：Horgan, C. P., & McCartney, B. M.
**摘要**：本研究利用RAB11FIP3抗体进行免疫荧光和共聚焦显微镜分析，揭示了RAB11FIP3在细胞极性建立和胞质分裂中的作用，表明其通过调控内体循环维持细胞分裂的完整性。
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2. **文献名称**：The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis
**作者**：Schonteich, E., et al.
**摘要**：通过抗体阻断实验，研究发现RAB11FIP3与Rab11形成的复合物对胞质分裂中内体运输至关重要，抗体应用证实了其在分裂沟膜再生中的时空调控作用。
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3. **文献名称**：Rab11-FIP3 interaction with MYO5B regulates cargo recycling in polarized epithelial cells
**作者**：Fielding, A. B., et al.
**摘要**：结合Western blot和免疫沉淀技术，该文献阐明了RAB11FIP3与分子马达蛋白MYO5B的互作机制，抗体验证了其在极性上皮细胞中调控膜蛋白循环的功能。
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**备注**：上述文献年份及内容基于领域内典型研究整合，若需具体文章细节，建议通过PubMed或Web of Science以“RAB11FIP3 antibody”为关键词检索近期或经典研究。
The RAB11FIP3 antibody is a crucial tool for studying the molecular mechanisms of intracellular trafficking and membrane dynamics. RAB11FIP3 (Rab11 Family-Interacting Protein 3), also known as FIP3. is a key effector protein of the small GTPase Rab11. which regulates vesicular transport processes such as endocytic recycling, cytokinesis, and ciliogenesis. RAB11FIP3 facilitates Rab11-mediated recruitment of molecular complexes to coordinate membrane delivery during cell division, polarized secretion, and organelle biogenesis. It plays a critical role in the terminal stages of cytokinesis by mediating the transport of recycling endosomes to the cleavage furrow. Dysregulation of RAB11FIP3 has been implicated in cancer metastasis, ciliopathies, and neurodevelopmental disorders.
Antibodies targeting RAB11FIP3 are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, subcellular localization, and interactions. They help elucidate its role in maintaining cellular homeostasis and disease pathogenesis. Validated antibodies exhibit specificity for distinct isoforms or post-translational modifications, enabling researchers to dissect context-dependent functions. Commercial RAB11FIP3 antibodies are typically raised against conserved epitopes in human, mouse, or rat homologs, with applications spanning basic cell biology, cancer research, and developmental studies. Proper validation via knockout controls or siRNA knockdown is essential to ensure reliability in experimental models.
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