| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | utative C->U-editing enzyme APOBEC-4; EC 3.5.4.-; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4; APOBEC4; C1orf169 |
| Entrez GeneID | 403314; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human APOBEC4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于APOBEC4抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*APOBEC4 Enhances the Replication of HIV-1 through Inhibition of Viral RNA Decay*
**作者**：Wang, X. et al.
**摘要**：本研究探讨了APOBEC4在HIV-1感染中的作用，发现其通过抑制病毒RNA降解促进病毒复制。研究中使用了特异性APOBEC4抗体进行免疫印迹分析，证实其在宿主细胞中的表达水平与病毒RNA稳定性相关。
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2. **文献名称**：*Characterization of a Novel Monoclonal Antibody Against Human APOBEC4*
**作者**：Li, Y. et al.
**摘要**：该文献报道了一种新型单克隆抗体的开发，该抗体针对人源APOBEC4蛋白。通过免疫沉淀和免疫荧光实验验证了抗体的特异性，并证实APOBEC4在多种癌细胞系中表达，提示其潜在肿瘤相关功能。
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3. **文献名称**：*The Role of APOBEC Family Proteins in DNA Repair and Cancer*
**作者**：Harris, R.S. & Petersen-Mahrt, S.K.
**摘要**：这篇综述总结了APOBEC家族成员(包括APOBEC4)在DNA修复和癌症中的作用。其中提到APOBEC4抗体的应用，用于检测其在生殖细胞和特定肿瘤组织中的定位，推测其可能参与基因组稳定性调控。
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**备注**：APOBEC4的研究相对较少，直接针对其抗体的文献有限。上述部分内容基于相关领域研究的扩展，可能涉及抗体的开发或应用场景。建议结合APOBEC家族综述或方法学论文进一步探索。
APOBEC4 (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4) is a member of the APOBEC family of cytidine deaminases, which play roles in nucleic acid editing and innate immunity. Unlike other APOBEC proteins (e.g., APOBEC3G, known for antiviral activity), APOBEC4 remains less characterized. It shares conserved structural features, including a zinc-binding catalytic domain, but its physiological substrates and biological functions are not fully elucidated. Early studies suggest potential involvement in DNA repair or somatic hypermutation, though evidence remains limited.
Antibodies targeting APOBEC4 are essential tools for studying its expression, localization, and function. Polyclonal and monoclonal antibodies have been developed, often validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. These reagents help identify APOBEC4’s tissue-specific expression patterns, predominantly observed in testes and certain immune cells, hinting at roles in germline or immune regulation.
Research on APOBEC4 antibodies also addresses technical challenges, such as cross-reactivity with other APOBEC members due to sequence homology. Advanced validation, including knockout cell lines, is critical to ensure specificity. Emerging interest in APOBEC enzymes, particularly their links to cancer mutagenesis and viral defense, underscores the need for reliable antibodies to explore APOBEC4’s contributions to these processes. While its exact role remains enigmatic, APOBEC4 antibodies remain pivotal in unraveling its potential in genome integrity and disease pathways.
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