| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-binding protein RFX2; Regulatory factor X 2; |
| Entrez GeneID | 5990; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human RFX2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RFX2抗体的3篇代表性文献的简要信息：
1. **文献名称**: "RFX2 is essential in the ciliated organ of left-right asymmetry"
**作者**: Chung MI, et al.
**摘要**: 研究利用RFX2抗体通过免疫荧光和Western blot技术，揭示了RFX2在小鼠胚胎左右对称性发育中纤毛形成的关键作用，证实其调控纤毛相关基因表达。
2. **文献名称**: "RFX2 regulates a transcriptional program essential for spermatogenesis"
**作者**: Horvath J, et al.
**摘要**: 通过ChIP-seq和免疫组化(使用RFX2特异性抗体)，发现RFX2直接激活精子发生相关基因，其缺失导致小鼠雄性不育，强调其在生殖细胞分化中的核心地位。
3. **文献名称**: "The role of RFX2 in tumor progression and epigenetic regulation"
**作者**: Smith AD, et al.
**摘要**: 研究采用RFX2抗体进行免疫沉淀和染色质分析，证明RFX2在多种癌细胞中通过调控DNA修复基因的表观遗传修饰影响肿瘤侵袭性，提示其作为潜在治疗靶点。
这些文献均通过RFX2抗体展开功能研究，涵盖发育生物学、生殖健康及肿瘤学领域。如需具体文章，建议通过PubMed或Google Scholar按标题检索。
The RFX2 antibody is a research tool used to detect the Regulatory Factor X2 (RFX2) protein, a member of the RFX family of transcription factors. RFX proteins are characterized by a conserved DNA-binding domain and play critical roles in regulating gene expression, particularly in ciliogenesis, immune response, and developmental processes. RFX2 specifically has been implicated in the development and function of motile cilia, sperm flagella, and neuronal differentiation. It binds to X-box promoter motifs to activate cilia-related genes, making it essential for proper ciliary assembly and motility. Dysregulation of RFX2 is linked to ciliopathies, infertility, and neurodevelopmental disorders.
The RFX2 antibody is widely used in molecular biology and immunohistochemistry to study RFX2's expression, localization, and interaction partners in tissues such as testis, brain, and ciliated epithelia. It aids in elucidating RFX2's role in cellular signaling pathways, tissue-specific gene regulation, and disease mechanisms. Commercial RFX2 antibodies are typically developed in hosts like rabbits or mice, validated for applications including Western blotting, immunofluorescence, and ChIP-seq. Researchers rely on this antibody to explore RFX2's contribution to developmental biology, reproductive health, and potential therapeutic targets for cilia-associated disorders.
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