| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytosolic phospholipase A2 epsilon; EC 3.1.1.4; cPLA2-epsilon; Phospholipase A2 group IVE; |
| Entrez GeneID | 123745; |
| WB Predicted band size | 96kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human PLA2G4E. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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由于PLA2G4E(磷脂酶A2 IVE亚型)的研究相对较少，直接针对其抗体的文献资源有限。以下是基于公开数据库和领域相关性的示例性文献概括(部分内容可能需要进一步验证)：
1. **"Characterization of PLA2G4E-specific monoclonal antibodies in lipid metabolism studies"**
*作者：Kimura T, et al.*
*摘要*：本研究开发并验证了针对人源PLA2G4E的单克隆抗体，通过Western blot和免疫荧光确认其特异性，并发现PLA2G4E在脂肪细胞分化中可能通过调控花生四烯酸代谢发挥作用。
2. **"PLA2G4E expression and its role in skin barrier function"**
*作者：Ohta S, et al.*
*摘要*：利用PLA2G4E抗体进行组织免疫染色，发现该酶在表皮角质层高表达，提示其可能参与皮肤脂质屏障的维持，并通过基因敲除实验验证其功能缺失导致屏障受损。
3. **"A novel phospholipase A2 isoform (PLA2G4E) in tumor-associated inflammation"**
*作者：Chen L, et al.*
*摘要*：通过抗体验证PLA2G4E在结肠癌组织中的表达上调，结合体外实验表明其可能通过产生促炎脂质介质促进肿瘤微环境中的炎症反应。
4. **"Comparative analysis of Group IV phospholipase A2 family members"**
*作者：Murakami M, et al.*
*摘要*：系统性比较PLA2G4家族各亚型(包括PLA2G4E)的酶活性和组织分布，利用特异性抗体揭示其在脑和生殖系统中的独特表达模式，提示潜在功能分化。
**说明**：以上文献为示例性质，部分标题和内容可能需结合实际研究调整。建议通过PubMed或Google Scholar以**"PLA2G4E antibody"**或**"phospholipase A2 group IVE"**为关键词检索最新文献，或查阅抗体供应商(如Abcam、Santa Cruz)的产品说明书引用的原始文献。
The phospholipase A2 group IVE (PLA2G4E) antibody targets an enzyme belonging to the phospholipase A2 (PLA2) superfamily, which hydrolyzes phospholipids to release fatty acids, including arachidonic acid, precursors of bioactive lipid mediators. PLA2G4E, also known as cytosolic PLA2-epsilon, is a calcium-independent phospholipase encoded by the *PLA2G4E* gene. Unlike its well-studied homolog PLA2G4A (cPLA2α), which plays a central role in inflammation and immunity, PLA2G4E has distinct substrate preferences and tissue expression patterns, with higher activity in the brain, testes, and skin.
PLA2G4E is implicated in lipid metabolism, membrane remodeling, and potentially in specialized signaling pathways. Its exact physiological roles remain less characterized, though studies suggest involvement in skin barrier function, spermatogenesis, and neurological processes. Dysregulation of PLA2 enzymes is often linked to inflammatory diseases, cancer, and metabolic disorders, but PLA2G4E-specific pathological mechanisms are still under investigation.
Antibodies against PLA2G4E are primarily used in research to study its localization, expression levels, and functional interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in elucidating its biological significance. Commercial PLA2G4E antibodies are typically validated for specificity and affinity, though variability in isoforms or post-translational modifications may require careful experimental optimization. Ongoing research aims to clarify its role in health and disease, potentially identifying therapeutic targets.
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