| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 180 kDa protein downstream of CRK; dedicator of cytokinesis protein 1; DOC1; DOCK180; |
| Entrez GeneID | 1793; |
| WB Predicted band size | 215kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human DOCK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DOCK1抗体的示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **标题**: "DOCK1 regulates cellular migration through modulation of Rac1 activity in cancer cells"
**作者**: Smith A, et al.
**摘要**: 本研究利用DOCK1特异性抗体，通过Western blot和免疫荧光技术，揭示了DOCK1在乳腺癌细胞中通过激活Rac1信号通路促进细胞迁移的机制。抗体验证表明其在体外实验中的高特异性。
2. **标题**: "Role of DOCK1 in macrophage phagocytosis and inflammatory response"
**作者**: Lee B, et al.
**摘要**: 通过抗DOCK1抗体的免疫沉淀实验，作者发现DOCK1与PI3K通路相互作用，调控巨噬细胞的吞噬功能。研究还通过流式细胞术证实了DOCK1缺失对炎症反应的影响。
3. **标题**: "DOCK1 antibody-based profiling in cardiovascular disease models"
**作者**: Zhang Y, et al.
**摘要**: 使用DOCK1抗体对动脉粥样硬化模型的小鼠组织进行免疫组化分析，发现DOCK1在内皮细胞中高表达，并与血管平滑肌细胞增殖相关，提示其潜在病理作用。
4. **标题**: "Development and validation of a novel anti-DOCK1 monoclonal antibody for diagnostic applications"
**作者**: Tanaka K, et al.
**摘要**: 本文报道了一种新型DOCK1单克隆抗体的开发，通过ELISA和免疫印迹验证其灵敏度及特异性，并探讨其在结直肠癌组织诊断中的应用潜力。
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**建议**：实际文献需通过PubMed、Web of Science等平台以关键词“DOCK1 antibody”或“anti-DOCK1”检索，并筛选涉及抗体应用(如检测、功能研究)的论文。部分研究可能侧重分子机制，但未明确提及抗体开发或验证，需注意区分。
The DOCK1 antibody is a tool used to detect and study Dedicator of Cytokinesis 1 (DOCK1), a protein encoded by the *DOCK1* gene in humans. DOCK1 belongs to the DOCK-A subfamily of guanine nucleotide exchange factors (GEFs) that activate small GTPases, particularly Rac1 and Cdc42. by facilitating the exchange of GDP for GTP. These GTPases regulate cytoskeletal dynamics, cell migration, and signaling pathways critical for immune responses, neuronal development, and cancer metastasis. DOCK1 is notably involved in phagocytosis, cell adhesion, and membrane ruffling through its interaction with ELMO1/2 proteins, forming the DOCK1-ELMO complex, which promotes GTPase activation and downstream signaling.
DOCK1 antibodies are widely used in research to investigate its expression, localization, and function in various biological contexts. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence to visualize DOCK1 in tissues or cultured cells. Dysregulation of DOCK1 has been implicated in pathological conditions, including cancer progression, immune disorders, and neurological diseases. For example, elevated DOCK1 expression correlates with tumor invasiveness and poor prognosis in certain cancers. Additionally, studies in model organisms, like mice, highlight its role in embryonic development and immune cell trafficking. The antibody’s specificity and reliability are crucial for deciphering DOCK1’s molecular mechanisms and its potential as a therapeutic target.
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