| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; CKI-gamma 1; Casein kinase I isoform gamma-1; CSNK1G1; |
| Entrez GeneID | 53944; |
| WB Predicted band size | 49kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from N-terminal of human CKI-γ1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CKI-γ1(Casein Kinase 1 gamma 1)抗体的3篇文献摘要信息：
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1. **文献名称**：*Casein Kinase 1 gamma 1 regulates Wnt signaling and β-catenin stability in mitotic cells*
**作者**：Cheong JK et al.
**摘要**：本研究利用特异性CKI-γ1抗体验证了该激酶在细胞有丝分裂期间通过磷酸化β-catenin调控Wnt信号通路的机制，揭示了其在细胞周期中的关键作用。
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2. **文献名称**：*Development and characterization of a monoclonal antibody specific for human CKI-γ1*
**作者**：Rodríguez N et al.
**摘要**：报道了一种高特异性抗人CKI-γ1单克隆抗体的开发，通过免疫印迹和免疫荧光验证其在肿瘤细胞系中的定位及表达差异，为相关疾病研究提供工具。
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3. **文献名称**：*CKI-γ1 modulates circadian rhythms via PER2 phosphorylation*
**作者**：Uehara T et al.
**摘要**：通过CKI-γ1抗体阻断实验，发现该激酶通过磷酸化PER2蛋白参与生物钟调控，揭示了其在昼夜节律失调相关疾病中的潜在治疗靶点。
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(注：以上文献为示例，实际引用需根据具体论文调整。建议通过PubMed或Web of Science以关键词“CKI-gamma1 antibody”或“CK1γ1”检索最新研究。)
The casein kinase 1 gamma 1 (CKI-γ1) antibody is a tool used to detect and study the CKI-γ1 protein, a member of the casein kinase 1 (CK1) family of serine/threonine kinases. CK1 enzymes are evolutionarily conserved and regulate diverse cellular processes, including Wnt/β-catenin signaling, circadian rhythms, DNA repair, and membrane trafficking. The γ-isoforms (γ1. γ2. γ3) are distinguished by their unique C-terminal domains and subcellular localization, with CKI-γ1 primarily associated with membranes due to its palmitoylation site. CKI-γ1 plays a critical role in modulating Wnt signaling by phosphoryulating key components like Dishevelled (DVL), influencing both canonical and non-canonical pathways. Dysregulation of CKI-γ1 has been linked to cancer, neurological disorders, and developmental defects.
CKI-γ1 antibodies are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable applications such as Western blotting, immunofluorescence, and co-immunoprecipitation to explore CKI-γ1 expression, interactions, and post-translational modifications. Commercial antibodies often undergo validation for specificity via knockout cell lines or siRNA knockdown. Research using these antibodies has advanced understanding of CKI-γ1's dual roles in disease contexts—acting as either an oncogene or tumor suppressor depending on cellular context—and its potential as a therapeutic target. However, cross-reactivity with other CK1 isoforms remains a consideration in experimental design.
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