| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity protein kinase CLK4; EC 2.7.12.1; CDC-like kinase 4; CLK4; |
| Entrez GeneID | 57396; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human CLK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与CLK4抗体相关的示例文献(内容为模拟概括，仅供参考)：
1. **"Characterization of CLK4 kinase activity and its role in alternative splicing regulation"**
- 作者：Smith, J. et al.
- 摘要：本研究利用特异性CLK4抗体，通过免疫沉淀和体外激酶实验验证了CLK4对SR蛋白的磷酸化功能，揭示了其在pre-mRNA剪接中的调控作用。
2. **"CLK4 expression correlates with tumor progression in colorectal cancer"**
- 作者：Li, X. et al.
- 摘要：通过免疫组化(使用兔源CLK4多克隆抗体)分析结直肠癌组织样本，发现CLK4高表达与患者预后不良相关，提示其作为潜在治疗靶点。
3. **"Development of a selective CLK4 inhibitor and its biological evaluation using CLK4-specific antibodies"**
- 作者：Fujita, R. et al.
- 摘要：报道了一种新型CLK4小分子抑制剂，并通过Western blot(使用抗CLK4单克隆抗体)证实其选择性抑制CLK4而非其他CLK家族激酶。
**注意**：以上文献为示例，实际文献需通过PubMed、Google Scholar等数据库检索关键词“CLK4 antibody”、“CDC-like kinase 4”等获取。近年研究可能涉及CLK4在癌症、神经疾病或RNA代谢中的机制。
The CLK4 (CDC-like kinase 4) antibody is a tool used to study the CLK4 protein, a member of the CDC-like kinase family involved in regulating pre-mRNA splicing. CLK4. a dual-specificity kinase, phosphorylates serine/arginine-rich (SR) proteins, which are critical for spliceosome assembly and alternative splicing. This phosphorylation modulates SR protein localization and interactions, influencing gene expression patterns. CLK4 is implicated in cellular processes such as cell cycle regulation, stress response, and differentiation, with dysregulation linked to diseases like cancer and neurodegenerative disorders.
CLK4 antibodies are essential for detecting protein expression, post-translational modifications, and subcellular localization in experimental models. They are widely used in techniques including Western blotting, immunoprecipitation, and immunohistochemistry. Researchers utilize these antibodies to explore CLK4's role in splicing-related pathologies or developmental pathways. For instance, studies highlight CLK4 overexpression in certain cancers, suggesting its potential as a therapeutic target.
Antibodies targeting CLK4 are typically validated for specificity using knockout cell lines or siRNA knockdowns. Commercial CLK4 antibodies may be polyclonal or monoclonal, with applications optimized for specific sample types (e.g., tissues, cultured cells). Proper controls, such as isotype-matched IgG or peptide competition, are recommended to confirm signal specificity. Understanding CLK4's function through antibody-based research contributes to insights into splicing mechanisms and disease pathways, advancing fields like oncology and neurobiology.
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