Rabbit Polyclonal Bax(Ab-167) Antibody

The Bax (Ab-167) antibody is a widely used tool in apoptosis research, targeting the Bax protein, a pro-apoptotic member of the Bcl-2 family. Bax plays a critical role in mitochondrial outer membrane permeabilization (MOMP), a key step in the intrinsic apoptotic pathway. Under stress signals, Bax undergoes conformational activation, translocates to mitochondria, and forms oligomers that promote cytochrome c release, triggering caspase activation and cell death. The Ab-167 antibody, often raised against a specific epitope (e.g., amino acids 1-171 in human Bax), is designed to detect endogenous Bax proteins across various applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It is particularly valued for distinguishing between inactive cytosolic Bax and active mitochondrial Bax in studies of apoptosis regulation. Researchers use this antibody to explore Bax’s involvement in diseases like cancer, neurodegeneration, and chemotherapy resistance, where dysregulated apoptosis is a hallmark. Validation typically includes testing in Bax-deficient models to confirm specificity. By enabling precise detection of Bax expression and localization, Ab-167 contributes to understanding cellular stress responses, drug mechanisms, and therapeutic targeting of apoptosis pathways. Its reliability and broad applicability make it a staple in laboratories studying cell death mechanisms.