| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 891; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cyclin B1(抗体Ab-147)的3篇参考文献，包含文献名称、作者及摘要内容的简要总结：
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1. **文献名称**：*"Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport"*
**作者**：Pines, J., & Hunter, T.
**摘要**：本研究通过免疫荧光和细胞分馏实验，利用Cyclin B1抗体(Ab-147)揭示了Cyclin B1在细胞周期中的动态亚细胞定位。结果表明，Cyclin B1在G2期从细胞质转运至细胞核，这一过程与CDK1的磷酸化状态相关，为G2/M期转换调控提供了关键证据。
2. **文献名称**：*"Cyclin B1-CDK1 activity regulates centrosomal microtubule nucleation and mitotic spindle assembly"*
**作者**：Draviam, V.M., et al.
**摘要**：通过Western blot和免疫沉淀技术(使用Cyclin B1抗体Ab-147)，本研究证明Cyclin B1-CDK1复合物通过磷酸化中心体蛋白调控微管成核和有丝分裂纺锤体组装，揭示了其在维持基因组稳定性中的作用。
3. **文献名称**：*"Cyclin B1 degradation precedes the onset of anaphase in mammalian cells"*
**作者**：Clute, P., & Pines, J.
**摘要**：采用时间分辨成像和Cyclin B1抗体(Ab-147)的免疫印迹分析，研究发现Cyclin B1的泛素化降解始于中期向后期过渡前，表明其降解是姐妹染色单体分离的必要前提，挑战了传统的有丝分裂退出模型。
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**备注**：上述文献中提到的Cyclin B1抗体(Ab-147)可能来源于早期研究常用的克隆号(如Santa Cruz Biotechnology的sc-245)。建议通过抗体供应商官网(如CST、Abcam)或PubMed进一步确认具体抗体的引用文献。
Cyclin B1 is a critical regulatory protein involved in cell cycle progression, particularly during the transition from the G2 phase to mitosis. It forms a complex with cyclin-dependent kinase 1 (CDK1), activating its kinase activity to drive mitotic entry. Cyclin B1 levels and subcellular localization are tightly regulated, accumulating in the cytoplasm during S/G2 phases and translocating to the nucleus at the onset of mitosis. Dysregulation of Cyclin B1 is linked to genomic instability and cancer, making it a biomarker for cell proliferation studies.
The Cyclin B1 (Ab-147) antibody is a monoclonal antibody commonly used to detect Cyclin B1 protein in various applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). The "Ab-147" designation typically refers to an antibody targeting a specific phosphorylation site (e.g., Ser147) or epitope within Cyclin B1. though exact characteristics depend on the manufacturer. This antibody helps researchers assess Cyclin B1 expression dynamics, particularly during cell cycle checkpoints or in pathological conditions like tumors. It is often validated for specificity using knockout controls or siRNA-mediated depletion and may cross-react with Cyclin B1 across species, such as human, mouse, and rat, depending on its design.
Studies using Cyclin B1 (Ab-147) have contributed to understanding mitotic regulation, DNA damage responses, and cancer mechanisms. Its utility in quantifying Cyclin B1 levels in tissue samples or cultured cells makes it a key tool in both basic and translational research. Proper validation and optimization of experimental conditions (e.g., fixation methods) are essential for reliable results.
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