| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/100 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAXI; PXN; |
| Entrez GeneID | 5829; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Paxillin (Ab-88)抗体的示例参考文献(内容为模拟，仅供参考)：
1. **文献名称**：*"Paxillin phosphorylation regulates cell migration and adhesion dynamics"*
**作者**：Smith A, et al.
**摘要**：研究利用Paxillin (Ab-88)抗体通过免疫荧光和Western blot分析，揭示了Paxillin在Tyr88位点的磷酸化如何调控细胞迁移和黏着斑的动态重组。
2. **文献名称**：*"Role of Paxillin in Integrin-Mediated Signaling Pathways"*
**作者**：Chen L, et al.
**摘要**：通过Paxillin (Ab-88)抗体的免疫沉淀实验，证实了Paxillin与整合素信号通路的相互作用，并探讨其在癌症转移中的潜在机制。
3. **文献名称**：*"Characterization of Paxillin Isoforms in Epithelial Cells"*
**作者**：Wang Y, et al.
**摘要**：使用Paxillin (Ab-88)抗体进行免疫组化，鉴定了不同Paxillin亚型在上皮细胞中的分布差异及其功能特异性。
4. **文献名称**：*"Paxillin Knockdown Impairs Focal Adhesion Formation in Fibroblasts"*
**作者**：Kim H, et al.
**摘要**：通过siRNA沉默Paxillin并结合Ab-88抗体的Western blot验证，发现Paxillin缺失会破坏成纤维细胞的黏着斑形成和细胞铺展。
**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar或抗体供应商(如CST、Santa Cruz)的引用列表检索。建议使用关键词“Paxillin antibody Ab-88”或抗体货号(如sc-5574)查询原始文献。
Paxillin is a multifunctional adaptor protein localized at focal adhesions, dynamic structures that mediate cell-extracellular matrix (ECM) interactions and regulate cell migration, survival, and signaling. It contains multiple protein-binding domains, including LD motifs and LIM domains, enabling interactions with cytoskeletal proteins (e.g., vinculin, talin) and signaling molecules (e.g., FAK, Src). Paxillin plays a critical role in integrin-mediated signaling, facilitating mechanotransduction and cytoskeletal remodeling during processes like wound healing and cancer metastasis.
The Paxillin (Ab-88) antibody is a specific monoclonal antibody targeting a conserved epitope near the Tyr88 phosphorylation site of human paxillin. This residue is critical for regulating paxillin’s interactions with downstream effectors upon integrin activation or growth factor stimulation. Ab-88 is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study paxillin expression, localization, and post-translational modifications in cell lines or tissue samples. Its specificity for phosphorylated Tyr88 makes it particularly valuable in investigating tyrosine kinase signaling pathways, cancer cell invasiveness, and ECM adhesion dynamics. Validated in multiple species, this antibody has become a key tool for exploring focal adhesion biology and related pathologies.
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