| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PLC-IV; PLC-gamma2; Phospholipase C-gamma-2 |
| Entrez GeneID | 5336; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCg2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLCγ2(Ab-1217)抗体的模拟参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**: *PLCγ2 in B Cell Signaling and Autoimmunity*
**作者**: Smith J, et al.
**摘要**: 本研究利用PLCγ2抗体(Ab-1217)通过免疫印迹和免疫组化分析，发现PLCγ2在B细胞受体信号通路中的关键作用。结果表明，PLCγ2缺失导致B细胞活化异常，与自身免疫疾病模型中的病理变化相关。
2. **文献名称**: *Role of PLCγ2 in Platelet Activation Pathways*
**作者**: Lee H, et al.
**摘要**: 通过Ab-1217抗体检测血小板中PLCγ2的表达及磷酸化状态，研究证实PLCγ2是胶原受体(GPVI)信号传导的核心分子，其活性异常与血栓性疾病风险增加相关。
3. **文献名称**: *Targeting PLCγ2 in Hematopoietic Malignancies*
**作者**: Garcia R, et al.
**摘要**: 使用Ab-1217抗体进行免疫沉淀和功能实验，发现白血病细胞中PLCγ2的过度激活促进细胞增殖。抑制PLCγ2可显著降低肿瘤生长，提示其作为治疗靶点的潜力。
4. **文献名称**: *PLCγ2 Antibody Validation for Immunoassays*
**作者**: Abcam Technical Note (非学术文献示例)
**摘要**: 该技术文档详述了Ab-1217抗体的特异性验证，包括在Western blot、流式细胞术中的优化条件及与PLCγ1的交叉反应测试，确认其适用于多种样本类型。
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**建议**：实际文献需通过 **PubMed**、**Google Scholar** 或抗体供应商官网(如Abcam、CST)检索，关键词可尝试“PLCγ2 antibody Ab-1217”或“Phospholipase C gamma 2 application”。若研究未明确提及抗体编号，需结合上下文判断。
The PLCγ2 (Ab-1217) antibody is a widely used tool in molecular biology and immunology research, targeting phospholipase C gamma 2 (PLCγ2), a key enzyme in intracellular signaling pathways. PLCγ2. a member of the phospholipase C family, plays a critical role in B-cell receptor (BCR) and Fc receptor (FcR) signaling, regulating processes like cell proliferation, differentiation, and immune response. It is activated by tyrosine phosphorylation upon receptor engagement, leading to hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers IP3 and DAG, which mediate calcium release and protein kinase C activation.
The Ab-1217 antibody, typically raised in rabbits as a polyclonal or monoclonal antibody, is validated for applications such as Western blotting (WB), immunoprecipitation (IP), and immunohistochemistry (IHC). It specifically recognizes PLCγ2 isoforms, distinguishing them from the closely related PLCγ1. and is often used to study B-cell malignancies, autoimmune disorders, and immune cell signaling. Researchers employ this antibody to investigate PLCγ2 expression patterns, activation states (e.g., phosphorylation at Tyr1217), and its role in diseases linked to dysregulated B-cell signaling, such as chronic lymphocytic leukemia (CLL) and rheumatoid arthritis. Its reliability in detecting endogenous PLCγ2 across human, mouse, and rat samples makes it a staple in both basic and translational research.
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