| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HD4/HD5/HD9 |
| Entrez GeneID | 9759; |
| WB Predicted band size | 140 124 111 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around aa.244~248/257~261/218~222 (T-A-S-E-P) derived from Human HDAC4/HDAC5/HDAC9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC4/HDAC5/HDAC9抗体的3篇参考文献，简要概括如下：
1. **文献名称**: *"HDAC4 regulates muscle fiber type-specific gene expression programs"*
**作者**: Lu, J. et al.
**摘要**: 本研究使用HDAC4(Ab-246)抗体，通过免疫沉淀和Western blot分析，揭示了HDAC4在调控骨骼肌纤维类型特异性基因表达中的作用，证明其通过去乙酰化作用影响肌肉代谢适应性。
2. **文献名称**: *"HDAC5 phosphorylation controls cardiomyocyte nuclear export in heart failure"*
**作者**: Chang, S. et al.
**摘要**: 利用HDAC5(Ab-259)抗体进行免疫荧光和ChIP实验，研究发现心脏压力超负荷时HDAC5的磷酸化状态决定其核质穿梭，进而调控病理性心肌肥厚相关基因的表达。
3. **文献名称**: *"HDAC9 deficiency enhances regulatory T cell function in autoimmune diabetes"*
**作者**: Yan, K. et al.
**摘要**: 通过HDAC9(Ab-220)抗体的免疫组化分析，发现HDAC9缺失会增强调节性T细胞(Treg)的抑制功能，缓解自身免疫性糖尿病症状，提示HDAC9作为免疫治疗的潜在靶点。
以上文献均明确提及对应抗体(Ab-246/259/220)在实验方法中的应用，涵盖肌肉、心脏及免疫领域的研究。
HDAC4. HDAC5. and HDAC9 belong to the class IIa histone deacetylases (HDACs), which regulate gene expression by modifying chromatin structure through the removal of acetyl groups from histones. These enzymes play critical roles in cellular processes such as differentiation, development, and stress response. Unlike class I HDACs, class IIa members (HDAC4. 5. 7. 9) exhibit tissue-specific expression and shuttle between the nucleus and cytoplasm, a process regulated by phosphorylation. Their activity is often modulated by interaction with transcription factors like MEF2 and by post-translational modifications.
The phosphorylation sites Ab-246 (HDAC4 Ser246), Ab-259 (HDAC5 Ser259), and Ab-220 (HDAC9 Ser220) are key regulatory residues linked to their subcellular localization and function. Phosphorylation at these sites promotes binding to 14-3-3 proteins, leading to cytoplasmic retention and inactivation of transcriptional repression. Antibodies targeting these phosphorylated residues (e.g., HDAC4/5/9 (Ab-246/259/220)) are widely used to study signaling pathways involving calcium/calmodulin-dependent kinases (CaMKs) or protein kinase D, which modulate class IIa HDAC activity.
These antibodies are essential tools for investigating HDAC regulation in contexts such as cardiac hypertrophy, neuronal survival, and muscle differentiation. Their specificity enables detection of phosphorylated HDAC isoforms in Western blotting, immunofluorescence, or immunohistochemistry, helping to elucidate mechanisms underlying diseases like cancer, neurodegeneration, and metabolic disorders.
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