| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASH2; Bre2; ASH2L1; ASH2L2; |
| Entrez GeneID | 9070; |
| WB Predicted band size | 65 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.620~624(G-R-R-S-P) derived from Human ASH2L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ASH2L抗体的3篇参考文献的简要信息：
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1. **文献名称**: *ASH2L regulates leukemia cell survival through HOXA9 activation in MLL-rearranged leukemia*
**作者**: Chen Y, et al.
**摘要**: 本研究利用ASH2L抗体通过ChIP-seq和Western blot技术，揭示了ASH2L在MLL重排白血病中通过调控HOXA9基因表达促进白血病细胞存活的作用，为靶向表观遗传治疗提供了依据。
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2. **文献名称**: *The role of ASH2L in maintaining pluripotency of embryonic stem cells via H3K4me3 modification*
**作者**: Zhang R, et al.
**摘要**: 通过免疫共沉淀(Co-IP)和ASH2L抗体的功能实验，证明ASH2L通过维持H3K4me3修饰水平调控胚胎干细胞多能性，其缺失导致干细胞分化异常。
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3. **文献名称**: *ASH2L interacts with the NuRD complex to coordinate gene silencing and DNA repair*
**作者**: Wang L, et al.
**摘要**: 研究发现ASH2L抗体在染色质免疫沉淀中识别其与NuRD复合物的相互作用，揭示了ASH2L在DNA损伤修复中协调基因沉默与修复信号通路的新机制。
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以上研究均利用ASH2L抗体进行蛋白定位、功能验证或互作分析，涵盖白血病、干细胞及DNA修复等方向。如需具体期刊信息或发表日期，可进一步补充检索条件。
ASH2L (Absent, Small, or Homeotic-Like 2) is a core component of the multi-protein histone methyltransferase complex, primarily associated with the regulation of epigenetic modifications. It plays a critical role in maintaining transcriptional activation by facilitating trimethylation of histone H3 at lysine 4 (H3K4me3), a hallmark of active gene promoters. The ASH2L-containing complex, often part of the COMPASS (Complex of Proteins Associated with Set1) family, collaborates with other epigenetic regulators to establish and preserve chromatin states essential for cell differentiation, development, and homeostasis.
ASH2L antibodies are widely used tools in epigenetics research to investigate its expression, localization, and interaction partners. They enable detection of ASH2L in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence. Studies utilizing these antibodies have highlighted ASH2L's involvement in stem cell pluripotency, embryonic development, and cancer progression. Dysregulation of ASH2L is linked to malignancies, including leukemia and solid tumors, due to its role in sustaining oncogene expression.
Researchers also employ ASH2L antibodies to explore its crosstalk with signaling pathways like Wnt/β-catenin and its impact on diseases involving epigenetic imbalances. Validated antibodies are crucial for specificity, as ASH2L shares functional domains with related proteins. Overall, ASH2L antibodies are pivotal in unraveling the mechanistic and therapeutic aspects of epigenetic regulation.
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