| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ICE-like apoptotic protease 6 |
| Entrez GeneID | 842; |
| WB Predicted band size | 47 37 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于Caspase 9抗体的模拟参考文献示例(内容基于公开研究概括，非真实文献)：
1. **文献名称**：*Caspase-9 Activation and Cleavage in Apoptosis Signaling*
**作者**：Li P, Nijhawan D, Budihardjo I, et al.
**摘要**：研究利用Caspase 9特异性抗体，通过Western blot和免疫沉淀技术，揭示了Caspase-9在细胞凋亡过程中被Apaf-1复合物激活的机制，并证实其裂解形式(cleaved Caspase-9)是凋亡标志物。
2. **文献名称**：*Antibody-based Detection of Caspase-9 Conformational Change during Apoptosis*
**作者**：Srinivasula SM, Ahmad M, Fernandes-Alnemri T, et al.
**摘要**：开发了一种特异性识别Caspase-9活性构象的单克隆抗体，用于流式细胞术和免疫荧光检测，证明其在凋亡早期Caspase-9构象变化的动态监测中的应用价值。
3. **文献名称**：*Role of Caspase-9 in Chemotherapy-induced Apoptosis: Studies with Caspase-9 Knockout Mice*
**作者**：Hakem R, Hakem A, Duncan GS, et al.
**摘要**：通过Caspase-9抗体进行组织免疫组化分析，发现Caspase-9缺失小鼠对化疗药物诱导的凋亡抵抗，表明Caspase-9在治疗相关细胞死亡中的核心作用。
4. **文献名称**：*A Novel Caspase-9 Antibody for Distinguishing between Inactive and Active States in Human Tissues*
**作者**：Zou H, Henzel WJ, Liu X, et al.
**摘要**：报道了一种高特异性多克隆抗体，可区分Caspase-9酶原(pro-Caspase-9)及其活性片段，应用于癌症组织样本中凋亡通路的活性评估。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索确认。)
Caspase-9 is a critical cysteine-aspartic protease involved in the intrinsic apoptotic pathway, playing a central role in programmed cell death. As an initiator caspase, it is activated within the apoptosome complex following mitochondrial outer membrane permeabilization (MOMP), triggered by cellular stress signals such as DNA damage or growth factor withdrawal. Active caspase-9 cleaves and activates downstream effector caspases (e.g., caspase-3/7), leading to apoptosis execution.
Caspase-9 antibodies are essential tools for detecting and quantifying caspase-9 expression, activation, and cleavage in research. These antibodies target specific epitopes, distinguishing between the inactive zymogen (procaspase-9. ~46 kDa) and its cleaved, active forms (~35/37 kDa). They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study apoptosis mechanisms in cancer, neurodegenerative diseases, and developmental biology. Caspase-9 dysregulation is linked to pathological conditions, including tumor resistance to chemotherapy and autoimmune disorders. Researchers also utilize these antibodies to evaluate therapeutic interventions targeting apoptotic pathways. Validation of caspase-9 antibodies is crucial, as cross-reactivity with other caspases or isoforms may occur. Proper controls, such as knockout cell lines or recombinant proteins, ensure specificity in experimental models.
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